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Journal of Applied Horticulture Selected Contents of Year
Arumugam Nithyanandam1*, T. Saraswathi2, C. Indu Rani1,L. Pugalendhi1, N. Manivannan3, S. Harish4 and N. Manikanda Boopathi5
1Department of Vegetable Science, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore – 641 037. India. 2Department of Medicinal and Aromatic Crops, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore – 641 037, India. 3Centre of Excellence in Molecular Breeding, Centre for Plant Breeding and Genetics, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore – 641 037, India. 4Department of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore –641 037, India. 5Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, (CPMB&B), Tamil Nadu Agricultural University, Coimbatore – 641 037, India
Abstract: The tomato represents an extensively cultivated crop within tropical and subtropical regions of the world for their fresh market and processing attribute. However, its production frequently encounters substantial setbacks due to notable losses associated with diseases such as Tomato Yellow Leaf Curl Virus (TyLCV). To address this challenge, the current study aimed to assess the resistance or susceptibility of selected 25 tomato breeding lines for TyLCV under natural field screenings to mimic real-world scenarios in accordance with the disease reaction score of Banerjee and Kalloo (1987). The field screening results showed that, the check Arka Vishes line demonstrated high resistance (HR) to TLCV, with reduced PDS, PDI values and a low coefficient of infection (CI). Several lines, including CBE SL 101, CBE SL 105, CBE SL 108, CBE SL 110, and CBE SL 114, exhibited a resistant (R) reaction, while others, such as CBE SL 102, CBE SL 107, CBE SL 112, CBE SL 120, and Arka Rakshak, displayed moderate resistance (MR). Conversely, lines CBE SL 103, CBE SL 104, CBE SL 106, CBE SL 109, CBE SL 111, CBE SL 115, CBE SL 117, and CBE SL 123 showed moderate susceptibility (MS), and the remaining lines, namely CBE SL 113, CBE SL 116, CBE SL 118, CBE SL 119, CBE SL 121, and CBE SL 122, were deemed susceptible to TLCV. The varying disease responses observed across these lines provide valuable insights into the complex dynamics of host-pathogen interactions in tomato plants, informing strategies for disease management and breeding efforts aimed at enhancing resistance to TLCV.
Abeer A.El.K. Soliman1 and Dalia M. Hassan2
1Vegetables Breeding Dept., Horticulture Research Institute, Agriculture Research Center, Giza, 12613 Egypt. 2Plant Protection Research. Institute, Agriculture Research Center, Giza, 12613, Egypt. Corresponding e-mail: bbsoliman76@hotmail.com
Abstract: An investigation was conducted at Kaha Vegetable Research Farm in the Kaliobia Governorate from 2021 to 2023 with an aim to evaluate the performance of six inbred lines of summer squash and their fifteen crosses in both open field and plastic greenhouse conditions. The study spanned two successive summer seasons (2022 and 2023) and focused on assessing horticultural traits and resistance levels to the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). Significant variations were observed in the mean performance of all traits among different genotypes. Analysis revealed significant differences in both general (GCA) and specific (SCA) combining abilities for all studied traits, except for the number of days to anthesis of the first female flower, indicating the presence of both additive and non-additive gene effects in traits inheritance. In the plastic greenhouse, genotype P3 exhibited the highest resistance with an average of 10.6 mites per 2 inches², while the cross P2 × P6 showed tolerance with an average of 4.4 mites per 2 inches². This study highlights the potential of new summer squash hybrids with desirable horticultural traits and resistance to the two-spotted spider mite. Additionally, parent 222/2 (P1) demonstrated the best combining ability for all traits, while parents 264 (P5) and 240/3 (P4) were the most effective combiners for total yield. Breeders may leverage these parents for genetic enhancement and the production of hybrids with superior horticultural qualities. Overall, this research provides valuable insights for researchers, breeders and farmers involved in the cultivation, breeding and utilization of summer squash in Egypt.
Taghreed E. Eissa1*, Hassan A. Elfeky1, Alaa El deen Shaheen2, Feng Xu 3,4* and Mohamed A.A. Ahmed5*
1Ornamental Plants and Landscape Gardening Research Department, Horticulture Research Institute, Agricultural Research Center, Giza, Egypt. 2Sandy and Limestone Lands Department, Water and Environment Research Institute, Agricultural Research Center, Giza, Egypt. 3Key Laboratory for Flower Breeding of Yunnan Province, National Engineering Research Center for Ornamental Horticulture, Floriculture Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, China. 4Yunnan Seed Laboratory, Kunming 650200, China. 5Plant Production Department (Horticulture-Medicinal and Aromatic Plants), Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt. Corresponding e-mail: dr.taghreed.eissa.2019@gmail.com; 148422486@qq.com; drmohamedmarey19@alexu.edu.eg
Abstract: This study aimed to investigate the impact of various fertilization methods and doses on the growth, flowering, and chemical composition of Iris tingitana cv. Wedgewood plants. The research aimed to address challenges hindering Iris plant development and bulb production, ultimately striving to cultivate high-quality plants suitable for Egyptian conditions. Conducted over two seasons (2021-2022 and 2022-2023) at the Horticulture Research Institute’s nursery of the Agriculture Research Center in Giza, Egypt, the experiment followed a factorial design with a randomized complete design (RCD) and three replicates. Results revealed that plants treated with 5 g/pot of phosphorein and 2 g/pot of kristalon, with irrigation intervals every 10 days after sowing (DAS), exhibited superior growth parameters. These included increased vegetative growth in terms of height, stem length, number of leaves per plant, fresh weight of cut spikes, diameter of the spike stem, and earliest flowering compared to other treatments across both seasons. Conversely, the application of 2 g/pot of kristalon and nitrobine, combined with irrigation intervals every 10 DAS, ranked second in enhancing plant growth and flowering characteristics in both seasons.
Dawn C.P. Ambrose1*, Ravindra Naik1 and Hrishikesh Patil1
1Central Institute of Agricultural Engineering, Regional Station, Coimbatore 641007, India. Corresponding e-mail: dawncp@yahoo.com
DOI: https://doi.org/10.37855/jah.2024.v26i02.34
Key words: Red chilli, with stalk, without stalk, drying, quality
Abstract: Processing of chilli is an important aspect in the production of good quality chilli. Harvested chilli has moisture content about 80% (w.b) which has to be properly dried to keep away from aflatoxin infestation. Open yard sun drying is the conventional method being practised which takes long time for drying. Pre-treatment like destalking could reduce the drying time considerably resulting in uniformly dried product. The present work was aimed to find the effect of drying on the quality of chilli. Red chilli (variety: Sannam) was dried with stalk and without stalk under open sun and solar tunnel dryer. The drying behaviour of chilli was studied. The influence of drying on the quality parameters viz., vitamin C content, colour (L*, a*, b* values), shrinkage and ASTA colour was found. Drying studies resulted in reduction in moisture content from 323% (d.b) to 8% (d.b). The time taken for drying under open sun drying and solar drying was 33 and 17 hours respectively for chilli dried with stalk. Similarly, the time taken for drying chilli without stalk under open sun drying and solar drying was 29 and 16 hours respectively. Quality analysis of dried samples showed higher vit.C (13 mg/100g) and ASTA colour value (67.4) for solar dried chilli without stalk.
D. Sarkar1, I. Sarkar*1, S. Chettri1 and S. Ojha2
1Department of Floriculture, Medicinal and Aromatic Plants, Faculty of Horticulture, Uttar Banga Krishi Viswavidyalaya, Pundibari, Cooch Behar, West Bengal-736165, India. 2Department of Agricultural Statistics, Faculty of Agriculture, Uttar Banga Krishi Viswavidyalaya, Pundibari, Cooch Behar, West Bengal-736165, India. Corresponding e-mail: indrajitsarkar_kpg@yahoo.co.in
Abstract: This study aimed to develop an effective in vitro protocol for germinating and multiplying Dendrobium moschatum seeds by identifying optimal concentrations of plant growth regulators for shoot proliferation and root formation. Five different MS media formulations were utilized, including a control (M?) and those supplemented with varying concentrations of kinetin and NAA (M?, M?, M?) and BAP (M?). Results showed significant variation in seed germination time across media, with the longest duration observed in M? (76 days) and the shortest in M? (55 days). Protocorm formation and plantlet development were quickest in M?, while M? and M? exhibited the longest duration. The initiation of leaves or shoots occurred earliest in M? (103 days) and latest in M? (149 days). Notably, leaf and plantlet growth differed between three- and five-month intervals, with M? demonstrating optimal growth over the longer term. These findings underscore the importance of carefully balanced plant growth regulator concentrations in achieving successful seed germination and subsequent growth in D. moschatum.
Suranjan Patowary* and Amiya Sarma
1Department of Economics, Gauhati University, Guwahati-781014 (Assam), India. Corresponding e-mail: patowarysuranjan@gmail.com
Abstract: The objective of the present study was to examine the seasonal and trend patterns in wholesale prices of tomato, pumpkin and cabbage over different seasons in Guwahati city, Assam. From time series and box plots, the study found that pumpkin prices are higher in November rather than lower prices during April or June. We find that cabbage is priced lowest from January to May and induces peaks in prices, rising the most in October. During January-April, tomato prices fall and reach a low point in April. From May onwards, the prices increase and peak in November. We performed the Mann-Kendal trend test, which showed no significant trend in the prices of all three perishable crops. It was proven that it is weakly loading and uniform across all years. Seasonal indices show a rise in the prices of pumpkins from May to October, cabbage from April to October and tomatoes from April to October reflecting their shorter shelf life and seasonal nature of these crops.
B. Keerthika1*, M. Thilagavathi1, C. Indu Rani2, M. Prahadeeswaran1 and R. Vasanthi3
1Department of Agricultural Economics, CARDS, TNAU, Coimbatore (641 003), India. 2Dept. of Vegetable Science, TNAU, Coimbatore (641 003), India. 3Department of Social Sciences, HC&RI, Periyakulam (625601), India. Corresponding e-mail: keerthikakural@gmail.com
DOI: https://doi.org/10.37855/jah.2024.v26i02.37
Key words: Cuddy-Della, concentration, instability, growth and trend
Abstract: India is one of the world’s leading fruit and vegetable producers after China owing to its diverse agro-climatic conditions. However, the 20-30 percent of the produce is wasted because of its perishability. The processed form of fruits is the most convenient for storage and export owing to its demand in foreign countries. Hence, this study analyzed the growth and instability of different processed fruit exports in India from 2001-02 to 2022-23. The Compound Annual Growth rates showed that exports increased overall growth rate, with a higher growth rate for jam, jelly and marmalades at 12 percent, followed by dried fruits (9 percent) and mango pulp (0.08 percent) in quantity exported. The instability was calculated using the Cuddy-Della Index for processed fruits’ export values (MT). It was realized from the result that the mango pulp category showed high instability in the overall export with Cuddy-Della Index value of 26.16 percent. In contrast, jam, jelly, marmalades, and dried fruits showed instability of 23.86 percent and 24.8 percent, respectively. This study concluded that proper infrastructure, cold chain, and supply chain for horticultural commodities should be developed for destination-specific production of processed fruits, as this could lead India to compete with global exporters and explore new alternate markets for the produce.
K. Boontiang1*, T. Siritrakulsak1 and C. Nontaswatsri2
1Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Kantharawichai 44150, Thailand. 2Department of Horticulture, Faculty of Agricultural Production, Maejo University, Sansai 50290, Thailand. Corresponding e-mail: kriangsuk.b@msu.ac.th
Abstract: Curcuma alismatifolia Gagnep. is one of the most attractive tropical flowers exported from Thailand. However, distributing its rhizomes free of bacterial wilt is considered to be a critical quarantine issue. This study’s strategic approach focused on cultivating C. alismatifolia in modified substrate on raised-bed planting without contamination by the wilt Ralstonia solanacearum. The experiment was conducted in a completely randomized design with 4 treatments (10 replications per treatment). Significant differences of the physical and chemical properties of appropriate substrates were observed to be correlated with mature green Azolla added in compost. This treatment promoted maximum growth and development, compared to the control. Moderate infection of R. solanacearum was detected in the rhizomes of plants grown with soil collected from the same location of a previous in-ground bedding crop (SSL) mixed in substrates. Healthy plants and decontaminated rhizomes were found in C. alismatifolia grown in modified substrates without SSL. Implementing the findings of this research has the potential to support sustainable production for C. alismatifolia.
P. Sujanthiya1, A. Sankari2*, H. Usha Nandhini Devi3, M. Djanaguiraman4, R. Kalaiyarasi5 and G. Dheebakaran6
1Department of Vegetable Science, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, 641003, India. 2Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, 641003, India. 3Horticultural College and Research Institute for Women, Tamil Nadu Agricultural University, Trichy, 620009, India. 4Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore, 641003, India. 5Department of Oil Seeds, Tamil Nadu Agricultural University, Coimbatore, 641003, India. 6Agro climate research centre, Directorate of Crop Management, Tamil Nadu Agricultural University, Coimbatore, 641003, India. Corresponding e-mail: sankisatha2020@gmail.com
DOI: https://doi.org/10.37855/jah.2024.v26i02.39
Key words: GC-MS, phytochemicals, secondary metabolites, retention time
Abstract: Allium cepa, commonly known as onion, is renowned for its culinary and medicinal properties. This article investigates the phytochemical composition of four aggregatum onionvarieties using preliminary phytochemical screening and gas chromatography-mass spectrometry (GC-MS) analysis. The study reveals diverse metabolites, including hydrocarbons, aldehydes, terpenoids, and organosulfur compounds, with variations observed among the onion varieties. Significant proportions of compounds such as diisooctyl phthalate, hexanol, and nobilitin, possessing various pharmacological activities like antimicrobial and antioxidant properties, were identified. The research emphasizes the importance of understanding onion phytochemistry, particularly given its widespread cultivation and consumption, notably in regions like Tamil Nadu, India. The identified bioactive compounds shed light on different onion varieties’ unique attributes and potential health benefits. By elucidating onion bulb metabolite profiles using advanced techniques like GC-MS, this study contributes to understanding the phytochemical diversity within Allium cepa species. These findings lay the groundwork for future research into the medicinal and nutritional advantages of onions and support targeted breeding (selective breeding) programs to enhance health-promoting compounds in onion varieties. Overall, this study underscores onions’ significance as a culinary staple and a valuable source of diverse bioactive compounds with pharmacological potential.
K.K. Dhatt1 and Bolagam Ravikumar1&2
1Department of Floriculture and Landscaping, Punjab Agricultural University, Ludhiana, Punjab, India. 2Department of Horticulture, Lovely Professional University, Phagwara, Punjab, India.Corresponding e-mail: ravikumar-fl@pau.edu
DOI: https://doi.org/10.37855/jah.2024.v26i02.40
Key words: Gladiolus, mulch, weed control efficiency, soil moisture
Abstract: This study evaluated the effects of various mulch types on weed management, soil moisture conservation, and crop performance in Gladiolus hybridus cv. Punjab Glance at Punjab Agricultural University, Ludhiana. The experiment tested different coloured polythene mulches—black (25µ), silver on black (25µ), transparent (25µ), and white (50µ)—as well as paddy straw mulch at the rate of 10, 15, and 20 tonnes/ha, and pendimethalin (0.5 kg/ha). Results demonstrated that transparent and white polythene mulches promoted earlier sprouting, bud initiation, and flowering. Black polythene mulch yielded the tallest plants (90.30 cm) and longest spikes (75.31 cm), while white polythene (50µ) mulch produced the most florets per spike (12.39) and extended the flowering duration (15.60 days). Hand-weeding achieved the highest weed control efficiency (100%), closely followed by black polythene mulch (99.87%). Paddy straw mulch (1 t/ha) was the most effective for soil moisture conservation, with polythene mulches also performing well. Transparent (25µ) and white (50µ) polythene mulches recorded the highest average soil temperatures during the study.
Yamini Sharma, Ashna Acharya* and Rajesh Bhalla
Department of Floriculture & Landscape Architecture, Dr. YS Parmar University of Horticulture & Forestry, Nauni, Solan-173230, H.P, India. Corresponding e-mail: ashnaacharya1997@gmail.com
Abstract: Lawn performs best with minimum inputs when grown in mixtures using more than a single grass specie. A study was conducted under open field conditions to evaluate the performance of four lawn grass species as mono-stand and in mixtures under mid-hill conditions at the Experimental Farm of the Department of Floriculture and Landscape Architecture, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.) during March, 2020 to March, 2021. The experiment was laid out in a randomized block design with twelve treatments and three replications. Data was recorded at fortnight intervals for total weed count, chlorophyll content, number of mowing, and overall presentability just after turf establishment. It was observed that all turf grass mixtures, performed better than the monostands, during the entire course of the study. Turf grass mixture (50% ‘Agrostis stolonifera’ + 20% ‘Festuca rubra’ + 20% ‘Lolium perenne’ + 10% ‘Cynodon dactylon)’ required least number of mowing and was presentable throughout the year attaining highest presentability score of 22.98.
Abstract: LA hybrid lilium cv. ‘Masai’ bulbs were grown over two consecutive growth seasons in a net house to enhance the yield of high-quality bulbs and bulblets, utilizing both organic and inorganic nutrients. The experiment tested five levels of organic manure (FYM) (0, 10, 15, 20, and 25 t ha-1) and inorganic fertilizers (0, 40+30+25, 80+60+50, 120+90+75, and 160+120+100 N+P2O5+K2O kg ha-1). It assessed both individual and combined effects of nutrient sources, concluding that their combined application was most beneficial. Treatment T24, comprising 25 t FYM with 160 kg N, 120 kg P2O5, and 100 kg K2O ha-1, proved to be the most effective. The study revealed that treatment T24 resulted in the largest bulb circumference, the highest number of bulblets per plant, the greatest bulblet circumference, and the highest fresh and dry weights of both bulbs and bulblets per plant. It also led to the best relative growth rate (RGR) of the bulb, as well as the most efficient partitioning rates at both the flowering and harvest stages. Treatment T23 notably influenced the fresh and dry weights of the bulblets. The data suggest that T24 produces bulbs and bulblets of superior quality compared to other treatments, making it a viable choice for the commercial cultivation of quality LA hybrid lilium bulbs and bulblets in the plains of India.
R. Shanmugabhavatharani1*, T. Saraswathi 2, M. Kavitha1, N. Manivannan3, N. Seenivasan4 and S. Harish5
1Department of Vegetable Science, 2Department of Medicinal and Aromatic Crops, 3Department of Plant Breeding and Genetics, 4Department of Nematology, 5 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India. Corresponding e-mail: tharanisekar1214@gmail.com
Abstract: Tomatoes represent a highly diverse crop renowned for their nutritional properties and extensive cultivation. Their abundant reserves of vitamins, minerals, dietary fibres, essential amino acids, and organic acids make them crucial nutrient sources across various contexts. Particularly noteworthy are their substantial levels of vitamin A and C, essential minerals, and pigments such as beta-carotene and lycopene, highlighting their biotechnological importance. The exploration of tomatoes’ genetic potential is essential for harnessing their inherent variability in breeding endeavours. In this present investigation, 27 distinct tomato parents and hybrids underwent principal component analysis (PCA) based on twenty diverse traits related to both yield and quality. The analysis identified eight principal components, collectively accounting for 80.4% of the variance. Constructing a bi-plot using the initial two principal components revealed that parents and hybrids CBESL160, CBESL164, CBESL168, H4xH5, H1xH5, and H5xH7 exhibited significant genetic divergence, dispersing across all quadrants. These genetic variations, categorized within PC1 and PC2, are potentially valuable additions to initiatives aimed at boosting crop yields through breeding efforts. Several traits, including growth habit, leaflet dimensions, fruit characteristics, and soluble solids content, demonstrated notable and statistically significant associations with yield per plant. Engaging Agglomerative Hierarchical Clustering and PCA, parents and hybrids CBESL160, CBESL164, CBESL168, H4xH5, H1xH5, and H5xH7 emerged as the most diverse parents and hybrids, offering potential avenues for further advancements in breeding efforts.
Swarna Mahajan1, Minhazul Kashem Chowdhury1, Sohel Rana Mazumder1, Abul Hasnat M Solaiman1, Zerin Tasnim1, S.M. Anamul Arefin2 and Jasim Uddain*
1Department of Horticulture, Faculty of Agriculture, Sher-E-Bangla Agricultural University, Dhaka-1207, Bangladesh. 2Department of Genetics and Plant breeding, Faculty of Agriculture, Sher-E-Bangla Agricultural University, Dhaka-1207, Bangladesh. Corresponding e-mail: jasimhort@sau.edu.bd
DOI: https://doi.org/10.37855/jah.2024.v26i02.44
Key words: Microgreens, LED, light spectrums, control farming
Abstract: Carefully selecting the appropriate lighting is vital for indoor farming systems to ensure sustainable agriculture and the production of microgreens rich in health-beneficial phytochemicals. This study aimed to investigate the impact of various light spectrums on the growth and nutritional composition of microgreens. The experiment focused on a single factor: five different concentrations of LED lights, specifically White light (L1) at 100%, Red light (L2) at 100%, Blue light (L3) at 100%, Red and Blue light (L4) at a 70:30 ratio, and Red, Green, and Blue light (L5) at a 70:10:20 ratio. Four microgreen crops were used: Mustard (C1), Lettuce (C2), Radish (C3), and Broccoli (C4). The results showed that the hypocotyl lengths of C1, C2, C3, and C4 were higher under the L4 light treatment (70:30 Red and Blue), measuring 10.53 cm, 8.47 cm, 15.23 cm, and 11.43 cm, respectively. The shorter hypocotyl lengths of 7.67, 5.53, 11.2 and 7.73 cm were observed under the L1 (White light) condition. The greater fresh weights for C1, C2, C3, and C4 (0.1 kg each) and yields (0.115 kg, 0.110 kg, 0.135 kg, and 0.125 kg, respectively) were also obtained under the L4 light condition. The higher SPAD values for C1 (38.2 nm), C2 (16.9 nm), C3 (55.3 nm), and C4 (49.9 nm) were recorded with the L4 light treatment. Additional findings included potassium content for C1 (0.19%), C2 (0.19%), C3 (0.22%), and C4 (0.16%), and antioxidant capacity for C1 (0.22%), C2 (0.23%), C3 (0.19%), and C4 (0.18%). The higher gross income was achieved with the L4C1, L4C2, L4C3, and L4C4 treatments, while the lower was with the L1C1, L1C2, L1C3, and L1C4 treatments. The benefit-cost ratios were higher (4.1, 3.9, 4.9, and 4.5) for the L4C1, L4C2, L4C3, and L4C4 treatments, respectively. Therefore, a 70:30 Red and Blue light combination (L4) can be used profitably in indoor farming to maximize growth, yield, and nutritional content of microgreens.
Pradeep Manyam1* and V.B. Nargund2
1S.V. Agricultural College, Acharya N.G. Ranga Agricultural University, Tirupati, Andhra Pradesh-517502, India. 2University of Agricultural Sciences, Dharwad, Karnataka-580005, India. Corresponding e-mail: m.pradeep@angrau.ac.in
Abstract: Bacteriophages offer an effective biocontrol strategy, presenting an environmentally friendly alternative to combat the issues of antibiotic and copper-based bactericide resistance in bacterial phytopathogens. In this study, two phages targeting Ralstonia solanacearun (designated as ?Rspv1 and ?Rspv2) were identified as potential biocontrol agents against solanaceous wilt, a highly destructive disease causing significant economic losses globally. Both phages demonstrated resistance to chloroform, while ?Rspv1 exhibited a broader host range compared to ?Rspv2 across five isolates of R. solanaceraum. The replication cycle for ?Rspv2 was determined to be 90 min. for a single growth cycle, with a burst size of 85 PFU per cell. Morphologically, ?Rspv1 featured icosahedral heads with long tails, characteristic of the Myoviridae family, whereas ?Rspv2 displayed a filamentous particle shape typical of Inoviridae viruses. Additionally, a Random Amplified Polymorphic DNA (RAPD) analysis using four random primers revealed a diverse nature of the two phages, suggesting their potential utility in disease management strategies. This research unveils two novel virulent phages, and their characterization highlights promising for phage therapy with future investigations aim to field level evaluation against R. solanacearum.
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