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Coastal Research and Extension Center, Plant and Soil Sciences Department, Mississippi State University, Poplarville, MS, USA 39470, 1-601-403-8939. Agricultural Hall, Department of Horticulture, Oklahoma State University, Stillwater, OK, USA
DOI: https://doi.org/10.37855/jah.2015.v17i02.21
Key words: Disease control, disease severity, Guignardia bidwellii, interspecific hybrid, organic, vine stress, Vitis spp.
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Abstract: Black rot, caused by Guignardia bidwellii (Ellis) Viala and Ravaz, and bud cold hardiness are both management issues in eastern U.S. viticulture. Black rot infections lead to vine stress, resulting in premature defoliation and rotten fruit, potentially compromising cold acclimation of the vine. No studies have targeted bud cold hardiness in relation to severity of prior season black rot infection. Thus, in 2011, �Noiret�, a hybrid winegrape, was subjected to four black rot control treatments: conventional (C), organic 1 (O1), organic 2 (O2), and no spray (N). Leaves and fruit were scored for black rot severity. The O1 and N treatments had the highest level of leaf and fruit disease severity and were not significantly different. The C treatment had the least amount of leaf and fruit disease severity and the O2 treatment was intermediate and significantly different from the O1, N, and C treatments. Bud samples were taken in January, February, and March 2012 and exposed to subzero temperatures (-21 �C, -23 �C, -26 �C, -29 �C) in an ethylene glycolbath to assess if prior season black rot infection impacted primary bud hardiness. In January and March nearly all buds were still alive at -21�C and -23�C, but -29�C caused more damage. Black rot control treatments were not a statistically significant factor in the bud hardiness experiment. This could be due to black rot severity being below a critical threshold for impact or the vines had enough time to recover in late summer and fall to reach full mid-winter hardiness.
Biotechnology Department, College of Agricultural Sciences, University of Guilan, Rasht, Iran. Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran.
DOI: https://doi.org/10.37855/jah.2015.v17i02.22
Key words: Acetosyringon, vir gene induction, virus induce gene silencing, Citrus tristeza virus
Abstract: Citrus trees are widely grown in tropical and subtropical climates due to their luscious taste, nutritional and medical benefits. Citrus fruits are native to southeastern Asia and are among the oldest fruit crops domesticated by humans. Breeding programs including the incorporation of genetic resistance to pests and diseases are necessary in this crop. Citrus tristeza virus (CTV) is of particular importance due to its rapid epidemic resulting in severe plant damage. The present research was aimed at transforming Citrus aurantinum with a gene encoding virus coat protein from CTV through Agrobacterium-mediated transformation. P25 coat protein gene was identified and then isolated from different CTV strains. Two regions of the gene were conserved among the genera and subcloned as a single chimer into a pFGC5941 silencing vector. Epicotyls-originated explants of C. aurantium were transformed by EHA105 strain of Agrobacterium tumefaciens. Some of the effective factors in gene transformation were examined by inoculation methods with Agrobacterium such as Acetosyringon effect (0, 50, and 100 �M), inoculation time (5, 10, 15, 20, and 25 min), and co-cultivation period (1, 2, 3 and 4 days). Based on our results, maximum number of transformed plants (13.7%) were obtained under combined treatment of 50 �M acetosyringone after 15 min inoculation time and 2 days of co-cultivation with Agrobacterium. One of the advantages of the current protocol is regeneration of explants through direct organogenesis which avoid callus phase and consequently somaclonal variation.
Forestry College, Henan University of Science and Technology, Luoyang 471003, China.
DOI: https://doi.org/10.37855/jah.2015.v17i02.23
Key words: Radish, mutant, compound leaf, microstructure, SRAP
Abstract: Previously we have developed a method, which uses two criteria, ‘time to flower opening’ and ‘vase life’, for characterizing flower opening profiles in cut spray-type flowers of carnation. These two criteria were used to evaluate the activities of flower preservatives, which accelerate flower bud opening, resulting in shortening the time to flower opening, and delay senescence, resulting in extension of vase life. In the present study, we developed the third criterion ‘gross flower opening’ which characterizes the ability of flower buds to open. Using this criterion the activity of analogs of pyridinedicarboxylic acids was successfully evaluated in addition to the previously-reported evaluation of their activity of acceleration of flower bud opening and extension of vase life.
Agricultural Research Service, U.S. Horticultural Research Laboratory, 2001 South Rock Road Ft. Pierce, FL34945-3030, USA
DOI: https://doi.org/10.37855/jah.2015.v17i02.24
Key words: Water, nonionic surfactants, gelling agents, malachite green, sodium sulphate, Citrus sinensis L. Osbeck. x Poncirus trifoliata L. Raf., C. sunki Hort. ex Tanaka. x Poncirus trifoliata L. Raf., C. paradisi Macf., C. sinensis L. Osbeck
Abstract: The effect of various treatments on shoot organogenesis from seedling epicotyl explants from various scion and rootstock polyembryonic citrus types was determined. Treatments included water source, gelling agent, explant insertion, seed size, light intensity, malachite green, nonionic surfactants, and sodium sulphate. Tap water, with the highest levels of SO42-, Ca2+, K+, Mg2+, and Na+, resulted in the most shoots compared to the other 5 sources, suggesting a mineral nutrient effect. Carrageenan produced fewer shoots than agar and gellan gum. Explants inserted into the medium produced more shoots than those cultured on the surface, presumably because of better exposure to water and nutrients. Seed size, light intensity, malachite green, and sodium sulphate had no effect on the number of shoots regenerated. Triton X-100 at 0.1 % resulted in significantly fewer shoots; otherwise, nonionic surfactants had no effect.
Department of Soil Soil Science and Land Resources, Bogor Agricultural University, Indonesia. Geospatial Information Agency, Indonesia. Indonesian Agency for Agricultural Research and Development, Ministry of Agriculture, Indonesia.
DOI: https://doi.org/10.37855/jah.2015.v17i02.25
Key words: Geographic information system, horticultural crop, land evaluation, land use planning
Abstract: Cashews have a potential economic value for local people, and as a conservation plant that is appropriate for small islands, which usually have limited resource capacities. The research for this paper was conducted on Lombok Island, Indonesia with the objective to delineate the potential areas for cashew, based on land availability and land suitability. Land availability was analyzed by taking into account the land use and land cover maps interpreted from SPOT-6 imagery, a Forest Areas Status map and a map from the Official Spatial Land Use Plan. The evaluation of the land’s suitability for cashews was conducted at a land mapping unit resulting from a soil survey, carried out at a scale of 1:25,000. The suitability analysis was done using a maximum limitation method, where the suitability level was defined by the lowest soil characteristics which determined the plant’s requirements. The land evaluation criteria were established in previous research, which included this island as an area of criteria establishment. The research results show that the land on this island has suitability status for cashews ranging from S2 (moderately suitable) to N (not suitable). The limiting factors include water availability, nutrient retention, available nutrients and rooting media, some of which can be improved. According to the available and suitable land, an area of 4,075.6 ha can be assigned as first priority, 18,167.3 ha as second priority and 43,582.8 ha as third priority for cashew expansion areas.
Department of Horticulture, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom-73140, Thailand.
DOI: https://doi.org/10.37855/jah.2015.v17i02.26
Key words: Dendrobium, cytokinin; BA, inflorescence, pseudobulb, deformed flower
Abstract: Improvement of flower quality is a major concern which plays a part in the enhancement of the marketability of the Dendrobium cut flower. In this study, both synthetic cytokinins: N-(2-chloropyridin-4-yl)-N-phenylurea (CPPU) and N-6-benzyladenine (BA), were foliar sprayed at rates of: 1, 5 or 10 mg L-1 and 100, 200 or 400 mg L-1, respectively, on current pseudobulbs of the Dendrobium Sonia Earsakul with 45-50 cm in length. The treatments were applied thrice at fortnight intervals, prior to terminal bud initiation. The results revealed that an application of 10 mg L-1 CPPU significantly increased the numbers of inflorescence per pseudobulb (from 1.1 to 1.7 flowers), and the number of flower on an inflorescence was increased from 12.2 to 13.8 flowers. The length and the diameter of flower inflorescence, having 10 mg L-1 CPPU application, also increased from 49.4 cm to 55.1 cm, and 0.57 cm to 0.66 cm, respectively. In addition, the largest flower width and the highest fresh inflorescence weight were also obtained with application of 10 mg L-1 CPPU treatment. Despite the application of BA, at 400 mg L-1, enhancing the highest amount of flower counts of inflorescence (at 14.8 flowers), 33.3% of those inflorescence obtained at least one deformed flower. Overall, the results suggest that CPPU spray has a higher potential to elevate flowers, along with the inflorescence qualities of Dendrobium Sonia Earsakul. Furthermore, according to this study, CPPU has lower effects upon abnormal flower shapes, and their times of harvest.
Division of Post Harvest Technology, Division of Plant Physiology and Biochemistry, Indian Institute of Horticultural Research, Hessaraghatta Lake P.O., Bangalore-560089, India.
DOI: https://doi.org/10.37855/jah.2015.v17i02.27
Key words: Sapodilla, wine, yeast, phenolics, wine clarification, head space volatiles
Abstract: Process was standardized for preparation of fermented beverage from sapodilla (Manilkara achras (Mill) Foseberg). The starter culture using yeast strain Saccharomyces cerevisiae UCD 522 fermented juice from two sapodilla varieties viz., Cricket Ball and Oval, to obtain wines with 10.1-11.2 % alcohol, 0.44- 0.58 % acidity, 3.6-3.9 pH, 0.26-0.28 % residual sugar, 300-645 mg/L phenolics and <0.09 % volatile acidity in six to nine days at 18 °C. Retention of peel while pulping improved the phenolics level; but reduced the sensory quality of wine. Bentonite dosage and period required for clarification was optimized as 0.04 % for 14 days and 0.08 % for 21 days for production of wine from peeled fruits of Cricket Ball and Oval varieties, respectively. Sensory evaluation of dry, sweet, and flavored wines revealed the potential market acceptability of the wines. Head space volatile analysis showed the presence of new odorous compounds like esters and short chain fatty acids during vinification of sapodilla juice. Methoxy compounds and carbonyl fractions were less in the finished wine compared to natural juice.
Department of Floriculture and Landscaping, Faculty of Horticulture, Bidhan Chandra Krishi Viswavidyalaya (BCKV) Mohanpur, Nadia-741252, West Bengal, India.
DOI: https://doi.org/10.37855/jah.2015.v17i02.28
Key words: Carotene, Meteorological Standard Weeks, planting time, Tagetes erecta L. cv. Siracole.
Abstract: The investigation was carried out to evaluate the growth, flowering, yield and quality of African marigold cv. Siracole, as influenced by different planting dates. The crop planted on 9th June (T3) was found to have the highest plant height (96.93 cm). Maximum number of primary (5.3) and secondary (14.15) branches/plant, total fresh weight (502.00 g/plant), contribution by stem (385.00 g/plant) to the total fresh weight, higher dry (126.25 g/plant) matter accumulation and also the dry matter accumulation in stem per plant (98.00 g/plant) were found maximum with 12 April (T1) planting. The individual leaf area (4.73 sq cm) on 90 days after planting was significantly higher in the crop planted on 16 May (T2). It took minimum days (13.01 days) from visible bud to colour shown and bud emergence to full bloom (20.16 days), maximum diameter of individual flower (3.99cm) were found with 12 April (T1) planting. Heaviest flower (2.55 g) was recorded with October 12 (T7) planting. 16th May (T2) planting produced maximum number (7434.67) of flowers per plot (6.4 m2). Maximum carotene content was noted with 12th October (T7) planting. Crops planted between 50th MSW (T9) 2011 to 3rd MSW (T10) 2012 produced very less crop biomass, dry matter content and flower yield.
Department of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431004 (Maharashtra), India.
DOI: https://doi.org/10.37855/jah.2015.v17i02.29
Key words: Bee flora, floral calendar, honey flow period, dearth period, Apis dorsata, A. cerana indica, A. florea, A. mellifera.
Abstract: The study was conducted at Paithan taluka of Aurangabad district during October 2012–September 2013 to identify existing bee flora and to determine honey flow and dearth period to develop the floral calendar. The flowering plants were visited and observed for the presence of honey bees and their foraging activities. Plants were reported as bee foraging species when at least three honey bees had visited the flowers within the period of 10 minutes. The result revealed that 63 plant species were useful to honeybees as source of food, out of which 41 were wild and 22 were agro-horticultural plants. The identified flora was further grouped into nectar, pollen and both nectar and pollen supplying plants. Out of 41 wild bee plant species, 17 were nectar producing, 4 were pollen producing and 20 were both nectar and pollen producing. Results also revealed that out of 22 agriculture bee plant species, 6 were nectar producing, 5 were pollen producing and 11 were both nectar and pollen producing. Mid-October to mid-December was identified as honey flow period of the year, having number of flowering plants. Mid-May to mid-August was the critical dearth period with few flowering plants. Based on the availability of flora, major characteristics of these plant species, utility status and flowering duration, the bee floral calendar was developed for Paithan taluka of Aurangabad district. The result indicated that the area has rich bee flora and is suitable for commercial bee keeping. Paithan taluka has four honey bee species, viz., Apis dorsata, A. cerana indica, A. florea and A. mellifera. Among these, A. florea and A. dorsata were dominant bee species, whereas A. mellifera was introduced species and only few colonies of A. cerana indica were observed.
State Level Biotechnology Centre, Mahatma Phule Krishi Vidyapeeth, Rahuri-413722, Maharashtra, India.
DOI: https://doi.org/10.37855/jah.2015.v17i02.30
Key words: Fig, regeneration, genotype-specific, shoot tip culture, multiple shooting, rooting
Abstract: The present investigation was undertaken to develop an efficient in vitro regeneration protocol in four fig cultivars viz., Poona Fig, Brown Turkey, Conadria and Deanna. Highest shoot tip establishment was observed in Deanna (100 %), followed by Conadria (79.2 %) and Brown Turkey (76.7 %) on MS medium supplemented with 2.5 mg/L 6-benzylaminopurine (BAP), 0.5 mg/L gibberellic acid (GA3). Establishment of shoot tips was very poor in cultivar Poona Fig (11.7-13.3 %). Further inoculation of shoots on MS medium supplemented with 1.0 mg/L indole-3-butyric acid (IBA) resulted in both multiple shooting as well as rooting. Significant number of newly formed shoots were observed in Conadria (4.7) and Deanna (3.8) as against in Brown Turkey (1) and Poona Fig (0.6). Highest root induction was observed in Conadria (73.3 %), followed by Deanna (52.2 %), Brown Turkey (26.7 %) and Poona Fig (24.4 %). These results confirmed that the shoot bud establishment and multiple shoot induction in fig is highly genotype specific. As the response of popular cultivar Poona Fig to shoot tip culture was very poor, tender leaf explants were further used for regeneration study. Optimum regeneration was observed using MS medium supplemented with 4.0 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) for callusing; 7 mg/L thidiazuron (TDZ) and 0.25 mg/L ?-naphthalene acetic acid (NAA) for shooting and 1.0 mg/L IBA for rooting.
Central Institute of Agricultural Engineering, Regional Centre, Coimbatore-3, Tamil Nadu, India.
DOI: https://doi.org/10.37855/jah.2015.v17i02.31
Key words: Curry leaf, prepackaging, color scores, physiological weight loss, volatile oil
Abstract: Curry leaf, which is a leafy spice, used in Asian culinary has limited shelf life. Investigation was carried out to extend the shelf life of fresh curry leaf by prepackaging in different packaging materials i.e., polyethylene bags of 38 and 75 micron thickness, polypropylene bags of 20 and 38 micron thickness and stored under ambient (30?2?C) and refrigerated (5 ?1?C) conditions. It was found that prepackaging fresh and stripped curry leaf in polypropylene bag of 20 micron thickness with 0.1 % vent area of 5 mm diameter vent could prolong the keeping quality for 4 days under ambient storage. Also under refrigerated condition, under the same packaging treatment, the sample kept well for a period of 16 days in polyethylene bag of 75 micron thickness.
Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan.
DOI: https://doi.org/10.37855/jah.2015.v17i02.17
Key words: AP3, class B gene, double pistils, PaPI, PaTM6, PI, Prunus avium
Abstract: We isolated APETALA3 (AP3)-like and PISTILLATA (PI)-like cDNA clones called PaTM6 and PaPI from sweet cherry (Prunus avium). PaTM6 showed very high similarity to the TM6 lineage of AP3 of other Rosaceae species. PaTM6 contained three amino acid residues (F, T, M) within the MADS box and the (H/Q)YExM sequence near the K box, both of which are characteristic of the AP3 subfamily. A paleo AP3 motif was present at the C-terminal end of PaTM6. PaPI showed very high similarity to PI of other Rosaceae species. PaPI had the serine residue and the KHExL sequence within the MADS box and near the K box, respectively, both of which are characteristic of the PI subfamily. A PI motif was present at the C-terminal end of PaPI. Both PaTM6 and PaPI genes were expressed specifically in petals and stamens, the same expression patterns as those of class B MADS-box genes. These results indicated that PaTM6 and PaPI are homologues of AP3 and PI, respectively.
Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto 606-8522, Japan. Kyoto Prefectural Institute of Agricultural Biotechnology, Seika Town, Kyoto 619-0224, Japan.
DOI: https://doi.org/10.37855/jah.2015.v17i02.18
Key words: Flower bud opening, display value, pyridinedicarboxylic acids senescence, spray-type carnation, vase life.
Abstract: Previously we have developed a method, which uses two criteria, ‘time to flower opening’ and ‘vase life’, for characterizing flower opening profiles in cut spray-type flowers of carnation. These two criteria were used to evaluate the activities of flower preservatives, which accelerate flower bud opening, resulting in shortening the time to flower opening, and delay senescence, resulting in extension of vase life. In the present study, we developed the third criterion ‘gross flower opening’ which characterizes the ability of flower buds to open. Using this criterion the activity of analogs of pyridinedicarboxylic acids was successfully evaluated in addition to the previously-reported evaluation of their activity of acceleration of flower bud opening and extension of vase life.
Department of Agricultural and Biosystems Engineering, North Dakota State University, Fargo, USA, Natural Resource Program, North Dakota State University, Fargo, USA.
DOI: https://doi.org/10.37855/jah.2015.v17i02.19
Key words: Vineyards, sensors, variable rate technology (VRT), tree-sensing
Abstract: Sensors have been used to detect tree sizes for agrochemical and fertilizer applications in grape vineyards. Rugged and reliable sensors are required to measure the size and quality of tree canopy volume for variable rate fertilizer application. Real time sensing is important as size of the tree changes with time due to biological factors and management practices. This study evaluated ultrasonic sensor, optical sensor and a laser sensor for their sensing characteristics and field of view (FoV) in a range of conditions. The FoV was established by moving targets perpendicular to the centerline on both sides. The maximum sensig range of sensors varied from 6 to 8 m with ultrasonic sensor having the highest range. The beam widths for ultrasonic sensors were found to be wide (maximum 950 mm) whereas optical sensor has a narrow maximum beam width of 70 mm. The laser sensor has a sharp beam and did not work well in outdoor environment with plant materials. Statistical analysis was also done for sensors and found that P value is lower than 0.001 and R2 value closer to 1.0 which indicates significant better result in the vineyard for sensing characteristics.
Bioinformatics Centre, Division of Crop Improvement, Central Plantation Crops Research Institute, P.O. Kudlu, Kasaragod 671124, Kerala, India.
DOI: https://doi.org/10.37855/jah.2015.v17i01.03
Key words: miRNAs, RNA, gene expression, in silico, miRBase, coconut, leaf transcriptome
Abstract: Micro RNAs (miRNAs) are single stranded, small and non-coding endogenous RNA molecules, which control the gene expression at the post-transcriptional level either by suppression or degradation. Because of its highly conserved nature, in silico methods can be employed to predict novel miRNAs in plant species. By using previously known plant miRNAs available at miRBase, we predicted 16 miRNAs, which belongs to 11 miRNA families, and also targets for seven potential miRNAs in coconut leaf transcriptome. A majority of these seem to encode transcription factors. To the best of our knowledge, this is the first report of in silico prediction and characterization of miRNA from coconut. These findings form an useful resource for future research into miRNA prediction and function prediction in coconut and for studies on their experimental validation and functional analyses.
