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International Center for Biosaline Agriculture, P.O. Box 14660, Dubai, UAE.

DOI: https://doi.org/10.37855/jah.2011.v13i01.06

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Abstract: The performance of asparagus (Asparagus officinalis L.) under the desert conditions of the Arabian Peninsula was evaluated. Ten cultivars introduced from six countries were studied. Two months old seedlings were transplanted into field plots in February 2007 and spears produced in the second year were harvested over a period of four weeks. Significant differences were found among cultivars for the number and quality of spears. The average number of spears harvested per plant varied between 5.0 and 26.4 and the spear yield between 36.3 and 159.2 g plant^-1. The spear number and yield were highest in cultivar PI 277824. Seed yields from the first year of harvest varied from 29.7 to 136.2 g plant^-1 among cultivars. In many cultivars, spear yields obtained in the second year were comparable to the yields reported from the productive environments in the tropics. The results show that asparagus has considerable potential for cultivation under the desert conditions of the Arabian Peninsula.

Corresponding author : Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake PO. Bangalore-560089, Karnataka, India.

DOI: https://doi.org/10.37855/jah.2011.v13i01.01

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Abstract: Transformation of tomato with heterologous genes requires rapid and efficient transformation protocols. Agrobacterium mediated transformation protocol of tomato (Solanum lycopersicum L.) cv. 'Arka Vikas' using dreb1A gene under Rd29A promoter in pCAMBIA 2301 binary vector was optimized by varying parameters such as type of explant, type and concentrations of hormones. Hypocotyls were found to be the best explants for shoot regeneration in tomato compared to cotyledons with 53.2 and 22.8% shoot regeneration, respectively. In the shoot regeneration medium, 0.1 mgL⁴ IBA as a source of auxin gave nearly 50% higher shoot regeneration than IAA at similar concentration. With this protocol it was possible to obtain transformed plants within a period of 77 days with a high regeneration and transformation efficiency (34%) compared to over 120 days using earlier published protocols. The T₁ generation plants segregated in a 3:1 ratio for the transgene and Southern blot analysis of the selected plants had shown the transgene integration was at a single locus. With this method it is possible to rapidly and efficiently generate transgenic tomato plants.

National Botanical Research Institute, Rana Pratap Marg, Lucknow, U.P., India.

DOI: https://doi.org/10.37855/jah.2011.v13i01.07

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Abstract: The scarcity of fertile land for growing ornamental crops has received attention on the utilization of salt affected soils for floriculture. Two cultivars of Chrysanthemum morifolium viz. 'Flirt' and 'Jayanti' were evaluated under three salinity levels (EC 4, 6 and 8 dS/m) of irrigation water along with control (irrigating with non-saline water). The experiment consisting of eight treatments with three replications was conducted in earthen pots with randomized block design. The pots were filled with sandy loam soil and 20 days old rooted cuttings were planted @ three cuttings per pot. The growth parameters viz., plant height, number of buds and flowers per plant; fresh and dry weight as well as chlorophyll content increased significantly with increasing salinity levels. 'Jayanti' appeared to be more salt tolerant in comparison to 'Flirt' in their floral morphology as well as in productivity. Nitrogen and phosphorus concentrations were reduced in plants as salinity increased. There was a relatively high N concentration in 'Flirt' cultivar. The concentration of other nutrients such as potassium, calcium and magnesium increased significantly with increasing salinity levels. This increase in nutritional uptake did not show any detrimental effect of Na toxicity in the salinized plants. As a result, nutrient use efficiency of nitrogen and phosphorus was enhanced with increasing salinity levels. Growth and flower yield both indicated that C. morifolium which is a plant of halophytic origin can be raised successfully as an ornamental cash crop in moderate saline environment, particularly 'Jayanti' cultivar. However, its critical limit to higher salinity tolerance is yet to be worked out.

Department of Floriculture and Landscaping, ASPEE College of Horticulture and Forestry, Navsari Agricultural University, Navsari - 396 450. Department of Horticulture, College of Agriculture, Indira Gandhi Agricultural University, Raipur-492 006, India.

DOI: https://doi.org/10.37855/jah.2011.v13i01.08

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Abstract: Plant regeneration of Anthurium andraeanum cultivars 'Pumasillo' and 'Corallis' has been achieved through adventitious shoots formation from callus. Young brown leaf lamina was used as explants for indirect organogenesis. These were disinfected using sodium hypochlorite (5%), ethanol (70 %) and HgCl₂ (0.1%). The results showed that both the varieties responded differently to callus induction, callus proliferation and shoot initiation treatments. In 'Corallis', the maximum number of cultures initiated callus on Nitsch medium supplemented with 5 mgL^-1 adenine and 2 mgL^-1 BAP while in Pumasillo, maximum number of cultures initiated callus on modified MS medium supplemented with 5 mgL^-1 adenine and 3 mgL^-1 BAP. Maximum shoot regeneration was observed on modified MS supplemented with 0.01 mgL^-1 TDZ in both the cultivars. Rooting medium was (modified half-strength MS salts) supplemented with 0.5 mgL^-1 IBA and 0.04% active charcoals. The callusing frequency of 'Pumasillo' was higher as compared to 'Corallis'. Rooted plantlets were succesfully acclimatized in polythene covered plastic trays containing cocopeat. Later on the hardened plantlets were successfully transplanted to commercial potting medium (1:1:1:1 cocopeat: rice husk: sand: FYM ) and were transferred to poly house with survival rate of ninety per cent.

Laboratory of Applied Biology, St. Aloysius College, Mangalore-575003, Karnataka, India.

DOI: https://doi.org/10.37855/jah.2011.v13i01.09

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Abstract: An efficient micropropagation system via multiple shoot induction directly from seedlings of S. megalanthus was developed. Seeds were germinated on Murashigae and Skoog (MS) basal medium supplemented with 6-Benzyl amino purine (BAP). The shoot tips of the germinating seedlings started callusing when left on the same medium. After two weeks, the entire shoot apex portion of epicotyl gave rise to a friable callus, portions of which started becoming green and morphogenetic. The callus gave rise to multiple shoots. The highest number of shoots i.e. 18.6 was produced on MS medium supplemented with 1.5 mgL"¹ BAP. The shoots, 3-4 cm in length, when transferred to MS basal medium supplemented with 1.0 mgL^-1 IBA, rooted within one week.

Faculty of Meteorology, Environment and Arid Land Agriculture, Department of Arid Land Agriculture, King Abdulaziz University, P.O.Box. 80208, Jeddah 21589, Saudi Arabia.

DOI: https://doi.org/10.37855/jah.2011.v13i01.10

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Abstract: During 2008 and 2009 seasons, the effect of pollen grain-water suspension spray at different concentration on fruit setting, yield and quality of 'Helali' date palm cultivar growing under Hada-Alsham conditions, KSA was examined. The results showed that fruit set, bunch weight and total yield per tree were significantly higher when spray pollinated at 0.5, 1.0 and 1.5 g L^-1 than the traditional pollination. In this respect, there were no significant differences among spray pollination at 0.5, 1.0 and 1.5 g L^-1. However, spray pollination at 2.0 g L^-1 gave fruit set percentage similar to traditional pollination. At harvest, the Rutab percentage in bunches was not affected by the method of pollination. At both the Bisir and Rutab stages, fruit, flesh and seed weight, diameter and length were not significantly affected by any of the pollination treatments. The biochemical characteristics of fruit such as TSS, acidity, vitamin C, total phenols and soluble tannins were not significantly affected by any of the pollination treatments at both stages. The concentrations of TSS and acidity were higher at the Rutab than at the Bisir stage. However, the concentrations of vitamin C, total phenols and soluble tannins were greatly lower at the Rutab than at the Bisir stage. This study showed the possibility to pollinate 'Helali' date trees by pollen grain-water suspension spray with optimum amount of pollen grains with no further need for thinning especially under hot arid conditions. However, more research work is required to standardize the optimum concentration of pollen grain-water suspension and the response of each date palm cultivar to this pollination technique.

Department of Horticulture Science, College of Agriculture, Shahid Chamran University, Ahwaz, Iran.

DOI: https://doi.org/10.37855/jah.2011.v13i01.11

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Abstract: Laboratory studies were conduced to determine the effect of salinity and temperature on seed germination of zinnia plant with two temperature regimes (25 and 30 ^oC) and 5 levels of salinity (distilled water as control and 3, 6, 9 and 12 dSm^-1) in a factorial completely randomized design. Analysis of variance showed significant difference (P<0.05) between different levels of salinity, and salinity and temperature interactions on germination percentage, germination rate and length of rootlet. But temperature treatment only influenced rootlet length (P<0.05). Mean comparison of seed germination percentage showed that increasing salinity decreased the seed germination. The highest rootlet length was recorded in the control (8.273 cm) and the lowest (1.92 cm) was at 12 dSm^-1 of salinity. The effect of temperature on the germination percentage, germination rate and length of rootlet determined that the highest germination percentage and length of rootlet was at 25 ^oC temperature. The study on the interaction of temperature and salinity exhibited that highest percentage of germination, germination rate and length of rootlet were in control at 25?C, whereas it was lowest at 30?C and 12 dSm^-1 salinity.

School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiasna, Punjab -141004, India.

DOI: https://doi.org/10.37855/jah.2011.v13i01.12

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Abstract: This is the first report of antibacterial and growth promoting effectiveness of cefotaxime in banana tissue culture. Young suckers (3-13 cm diameter) of commercially important banana varieties viz., 'Grand Naine' and 'Robusta' were regenerated into shoots on MS-medium supplemented with 6-benzylaminopurine (BA, 5 mgL^-1) and multiplied on Banana Multiplication Medium (BMM) for 7 cycles of 3 weeks each. Shoots were separated and cultured on BMM supplemented with IBA (1.0 mgL^-1) and cefotaxime at various concentrations viz., 0, 100, 200, 300, 400, 500, 600 and 700 mgL¹. Maximum shoot multiplication and elongation with respect to number of shoots, shoot length and plantlet fresh weight in both the genotypes was obtained with cefotaxime used at the rate of 400 and 500 mgL^-1 in the medium. Among the different varieties, on the basis of mean of 50 shoot cultures, number of shoots per culture and shoot length was highest in cv. Robusta i.e. 7.20 (41.6% increase over control) and 8.50 cm (30.5% increase over control), respectively. Whereas, plantlet fresh weight was highest in cv. Grand Naine (494.8 mg, 38.9% increase over control) after 3 weeks of culturing with cefotaxime used at the rate of 500 mgL^-1. Results revealed significant differences among varieties and media for different shoot multiplication and elongation parameters. Therefore, use of cefotaxime during tissue culture and genetic transformation of banana can improve frequency of shoot multiplication and transformation, respectively.

University of Al Zaeim Al Azhari -Faculty of Agriculture, Khartoum North - P.O. Box 1432- Sudan

DOI: https://doi.org/10.37855/jah.2011.v13i01.13

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Abstract: Seven lines of okra Abelmoschus esculentus [L.] (MOENCH) were evaluated for general and specific combining ability using three diverse testers following a line x tester mating design as described by Kempthorne (1957). Twenty one F₁'s hybrids along with ten parental lines were raised at the Demonstration Farm of the Faculty of Agriculture, University of Al Zaeim Al Azhari, Sudan, using randomized complete block design with three replications. Analysis of variance revealed significant differences among genotypes existing for yield and associated traits (P=0.01), indicating the presence of sufficient genetic variability in the material studied. Significant variability existed among hybrids (P=0.01) for number of pods per plant, length of pod, pod yield per plant and 100 seeds weight. Estimation of general combining ability effect identified lines HSD 1835, HSD1840 and HSD 2550 as a good general combiners for pod dry weight (P=0.05). Among testers Sinnar was found to be a good general combiner for number of pods per plant, length of pod, yield per plant, number of seed per pod and 100 seeds weight. Estimation of specific combining ability effect identified hybrids HSD 2550 x Sinnar as the best parent combination for number of pods per plant and yield per plant. HSD2543 x Sinnar and HSD1840 x Clemson Spineless recorded as the best parental combination for length of pod. The additive as well as non-additive gene effects played significant role in the inheritance of yield and yield related traits with predominance of additive gene action in the inheritance of major yield contributing traits. Testers contribution percentage were significantly higher for number of pods per plant (77.04%), pod dry weight (40.06 %) and number of seeds per pod (45.04%). The percentage contributions of the interaction were significantly higher and evident in the rest of the traits.

Department of Agronomy, Faculty of Agricultural Sciences, Ladoke Akintola University of Technology, P.M.B 4000, Ogbomoso, Oyo State, Nigeria.

DOI: https://doi.org/10.37855/jah.2011.v13i01.14

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Abstract: Field experiments were conducted at the Teaching and Research Farm, Ladoke Akintola University of Technology, Ogbomoso (8^o 10'N and 4^o 10'E) to determine the effect of mulching materials and rates of NPK fertilizer application on the growth and yield of fluted pumpkin (Telfairia occidentalis). The trials consisted of three levels (0, 250 and 350 kg ha¹) NPK and four types of mulching materials (white polyethylene, black polyethylene, saw dust and Panicum grass). A factorial arrangement fitted into complete randomized block was used and replicated three times. Plant height and number of leaves increased as the NPK rate increased, irrespective of the mulching materials. The yield and yield components of telfairia increased as the NPK rate increased from 0 up to 250 kg ha^-1 and then declined at 350 kg NPK ha^-1. These were significantly (P<0.05) improved by the main effects of fertilizer and mulching materials. Although, the best performance of telfairia in term of yield and quality were obtained from white polyethylene mulching material, this was comparable with that of dry Panicum grass mulching material. Except for fibre and vitamin C contents, telfairia seeds contained higher quality attributes investigated under this study than leaves. Therefore, the yield and quality of telfairia could significantly be improved by the application of NPK fertilizer at the optimum rate of 250 kg ha^-1 with and without mulching.

International Center for Biosaline Agriculture (ICBA), P.O. Box 14660, Dubai, UAE.

DOI: https://doi.org/10.37855/jah.2011.v13i01.15

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Abstract: The performance of Gaillardia aristata Pursh, irrigated with saline water was studied with the objective to identify herbaceous ornamentals for saline landscapes. Seeds were sown directly into field plots of sandy soil and irrigated with saline water at electrical conductivity (EC_W) of 2 (control), 5, 10 and 15 dSm^-1. Increase in salinity reduced the plant stand by 38% at 5 dSm^-1, 50% at 10 dSm^-1 and 67% at 15 dSm^-1, in comparison with the control. Increased salinity also decreased the mean height, number of branches and dry weight of the plants, but the differences among treatments lower than 15 dSm^-1 salinity were statistically insignificant (P>0.05). Compared with the control, the number of flowers per plant declined by 57% at 5 dSm^-1, 61% at 10 dSm^-1 and 67% at 15 dSm^-1. The differences in flower production among 5, 10 and 15 dSm^-1 treatments were not significant (P>0.05). The flower diameter was significantly reduced at 15 dSm^-1, but was not affected at the lower salinities. The results show that G. aristata could be successfully cultivated upto irrigation water salinity level 10 dSm^-1 in sandy soils.

Department of Chemistry, University of Colombo, Colombo 03, Sri Lanka. Department of Crop Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka.

DOI: https://doi.org/10.37855/jah.2011.v13i01.16

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Abstract: Simplified hydroponics is a low cost aggregate hydroponics system which is practiced under natural climatic conditions with hand watering. Rice hull, a waste material, which is mostly under utilized and a mixture of rice hull and sand (3:2) was used in this system. A new nutrient formulation (NF) was developed using locally available commercial grade chemicals. A buffer system was incorporated to the nutrient formulation, ensuring that the pH was maintained in the optimum range. NF was composed of (in ppm), N = 167, P = 80, K = 281, Ca = 234, Mg = 57, S = 251, Cu = 0.01, Fe = 3.9, Zn = 0.13, Mn = 1.2 and Mo = 0.13. A field trial was carried out for NF in the wet zone in Sri Lanka for tomato plants in simplified hydroponics system. The nutrient uptake was monitored by measuring the changes in weekly average electrical conductivity (EC) of the root solution (compared to the EC of the applied nutrient) of tomato plant beds. The pH of the root solution was in the suitable range and no adjustment was required indicating sufficient buffering in the formulation. The nutrient cost involved to produce 1 kg of tomato was about 70% less than other commercially available nutrients formulation.

Division ofVegetable Crops, Indian Institute of Horticultural Research, Hesaraghatta Lake, Bangalore-560089,Karnataka, India.

DOI: https://doi.org/10.37855/jah.2011.v13i01.17

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Abstract: A field experiment was conducted during 2007-2010 to study the effect of levels of organic manure and conventional practices on growth and yield of French bean grown organically. The trial was carried out in organic experimental block of IIHR farm, Hesaraghatta, Bangalore. The trial included four levels of organic manure nutrient and two inorganic nutrient supply treatments. The treatment which received 100 per cent recommended dose of N (RDN) through organics produced the highest pod yield (17.77 t/ha) followed by treatments which received 75 per cent RDN through organics and conventional practices (17.45 and 15.93 t/ha). Plant growth parameters such as plant height (45.5 cm), number of leaves per plant (42.9), leaf area per plant (2706 cm²), nodules per plant (43.9), pod weight per plant (160.1g) and pod length (14.7 cm) were also comparatively higher in this treatment resulting in better pod yield. Application of recommended dose of chemical fertilizer in conjunction with farm yard manure recorded higher values for growth and yield parameters like number of leaves, leaf area, dry matter production, number of pods, pod weight per plant and pod length which also resulted in significantly higher pod yield as compared to the treatment which received recommended dose of chemical fertilizer only.

National Horticultural Research and Development Foundation, Chitegaon Phata, Post-Darna Sangavi, Taluqa-Niphad, Nashik, 422 003, Maharashtra, India.

DOI: https://doi.org/10.37855/jah.2011.v13i01.18

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Abstract: An investigation was carried out to study the genetic variability in late kharif germplasm of onion at Nashik, Maharashtra (India). The mean data indicated that the highest gross yield (41.17 t/ha) and marketable yield (39.13 t/ha) was recorded in line 744 and was at par with line 682 (39.07 t/ha) and (34.39 t/ha). A wide range of variability was observed for gross yield (19.65 to 41.17 t/ha), marketable yield (10.05 to 39.13 t/ha), bulb size index (20.40 to 35.90 cm²), bolters (0.00 to 40.83%), doubles (0.00 to 47.50 %), thrips/plant (8.75 to 25.80) and plant height (54.95 to 71.80 cm). A higher magnitude of coefficient of variation was recorded for bolters (112.78 -112.65%), followed by doubles (86.35-86.16%), thrips/plant (37.55-37.36) and marketable yield (29.34 and 29.90 %). Highest heritability was noted in doubles, gross yield, bulb diameter, plant height, bolters and thrips/plant. The genetic advance as percent of mean ranged from 3.93 to 231.73. High genetic advance noted in bolters (231.73 %), doubles (177.12 %), thrips/plant (76.56%) and marketable yield (54.53%) and rest of others characters showed medium to low genetic advance. Gross yield, marketable yield, doubles, bolters, thrips/plant and bulb size index indicated higher estimates of genetic advance as percent of mean coupled with high heritability, suggesting the involvement of additive genetic variance for these traits. Marketable yield was significantly and positively correlated with plant height, neck thickness, bulb diameter, bulb size index, weight of 20 bulbs, and gross yield and negatively correlated with bolters, doubles and days for bulb initiation at genotypic and phenotypic levels. Plant height, leaves/plant, bulb diameter, bulb size index, weight of 20 bulbs and days for bulbs initiation. The study revealed that a wide range of variability for important characters exists in germplasm offering a good scope for developing improved onion varieties suitable for cultivation in Maharashtra.

Department of Botany, University of Calabar, Calabar, Nigeria. Department of Soil Science, University of Calabar, Calabar, Nigeria.

DOI: https://doi.org/10.37855/jah.2011.v13i01.19

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Abstract: The effect of Glomus fasciculatum inoculation on the growth and yield of tomato (Solanum lycopersicum L.) in a Meloidogyne incognita infested soil was investigated in a pot experiment. G. fasciculatum greatly enhanced the growth and yield of tomato in both non-nematode and nematode infested soils. Growth was greatly impeded to the point of no flowering or fruiting in non-mycorrhizal nematode infested soil treatments. While the adverse effect of nematode to the growth of tomato can be attributed to impaired transport of water and nutrients, the enhanced growth in mycorrhizal plants was associated with increased water and nutrient uptake, resulting improved growth which must have conferred more vigour on them to reduce the pathogens' effect.