SELECTED CONTENTS

 Journal of Applied Horticulture Selected Contents of Year

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Paramesh, T H; Sona Chowdhury

Division of Ornamental Crops, Indian Institute of Horticultural Research, Hessaraghata, Bangalore - 560 089, India.

Key words: benzyladenine, callus, carnations, culture media, explants, gamma radiation, gibberellic acid, in vitro culture, in vitro regeneration, irradiation, leaves, micropropagation, mutagenesis, NAA, plant growth regulators, survival, thidiazuron

Journal of Applied Horticulture, 2005, volume 7, issue 1, pages 43-45.

Abstract: In vitro shootlets of carnation (cv. IIHRS-1) were subjected to irradiation with gamma dosage of 20, 40, 60 and 80 Gy. In vitro shootlets required for the irradiation were generated on MS media supplemented with 0.25 mg BAP [benzyladenine], 0.1 mg NAA and 0.25 gibberellic acid/l. From irradiated shootlets, leaves were excised and used as explants for further culturing. Leaves were horizontally cut into half. The region adhering to stem was considered as the leaf base and the region that is away from the stem was considered as the leaf tip. The leaf tip and leaf base were incubated on MS media supplemented with (1) 1.0 mg thidiazuron (TDZ) and 0.1 mg NAA/l (M5) and (2) 0.3 mg TDZ, 1.0 mg BAP and 0.1 mg NAA/l (M6). Weekly observations were recorded for survival percentage, callus formation, regenerated shootlets and expansion of leaf area. The results indicated gamma-radiation at 40 Gy to be the ideal dosage for mutagenesis when mutagenesis was used in combination with regeneration. Survival percentage decrease
Paramesh, T H; Sona Chowdhury

Division of Ornamental Crops, Indian Institute of Horticultural Research, Hessaraghata, Bangalore - 560 089, India.

Key words: benzyladenine, callus, carnations, culture media, explants, gamma radiation, gibberellic acid, in vitro culture, in vitro regeneration, irradiation, leaves, micropropagation, mutagenesis, NAA, plant growth regulators, survival, thidiazuron

Journal of Applied Horticulture, 2005, volume 7, issue 1, pages 43-45.

Abstract: In vitro shootlets of carnation (cv. IIHRS-1) were subjected to irradiation with gamma dosage of 20, 40, 60 and 80 Gy. In vitro shootlets required for the irradiation were generated on MS media supplemented with 0.25 mg BAP [benzyladenine], 0.1 mg NAA and 0.25 gibberellic acid/l. From irradiated shootlets, leaves were excised and used as explants for further culturing. Leaves were horizontally cut into half. The region adhering to stem was considered as the leaf base and the region that is away from the stem was considered as the leaf tip. The leaf tip and leaf base were incubated on MS media supplemented with (1) 1.0 mg thidiazuron (TDZ) and 0.1 mg NAA/l (M5) and (2) 0.3 mg TDZ, 1.0 mg BAP and 0.1 mg NAA/l (M6). Weekly observations were recorded for survival percentage, callus formation, regenerated shootlets and expansion of leaf area. The results indicated gamma-radiation at 40 Gy to be the ideal dosage for mutagenesis when mutagenesis was used in combination with regeneration. Survival percentage decrease
Singla, M L; Jain, S C; Shweta Sharma; Angra, S K

Central Scientific Instruments Organization, Sector 30-C, Chandigarh - 30, India.

Key words: absorbance, apples, cultivars, monitoring, nondestructive testing, plant disorders, techniques, transmission, water core

Journal of Applied Horticulture, 2005, volume 7, issue 1, pages 46-48.

Abstract: This paper presents the transmission technique, which has been designed and developed for the study of water core in apple cultivars Red Delicious and Golden Delicious of Himachal Pradesh, India. The principle of this method is to measure the optical density of the sample at 2 selected wavelengths and computation of the optical density difference. It has been observed that Golden Delicious is more prone to water core than Red Delicious. The technique is simple and can be conveniently implemented to develop an on-line instrument to monitor water core in apples.
Singla, M L; Jain, S C; Shweta Sharma; Angra, S K

Central Scientific Instruments Organization, Sector 30-C, Chandigarh - 30, India.

Key words: absorbance, apples, cultivars, monitoring, nondestructive testing, plant disorders, techniques, transmission, water core

Journal of Applied Horticulture, 2005, volume 7, issue 1, pages 46-48.

Abstract: This paper presents the transmission technique, which has been designed and developed for the study of water core in apple cultivars Red Delicious and Golden Delicious of Himachal Pradesh, India. The principle of this method is to measure the optical density of the sample at 2 selected wavelengths and computation of the optical density difference. It has been observed that Golden Delicious is more prone to water core than Red Delicious. The technique is simple and can be conveniently implemented to develop an on-line instrument to monitor water core in apples.
Rathinakumari, A C; Kumaran, G S; Mandhar, S C

Section of Agricultural Engineering, Indian Institute of Horticultural Research, Bangalore, Karnataka 560 089, India.

Key words: cabbages, cauliflowers, containers, design, drilling, drills, equipment performance, mechanization, performance tests, vegetable growing, vegetables, work capacity

Journal of Applied Horticulture, 2005, volume 7, issue 1, pages 49-51.

Abstract: A tray type dibbler with capacity of 100 portrays/h and a vacuum seeder with capacity of 50 portrays/h were designed and developed. The tray type dibbler is made out of wooden board with 98 nylon pegs to dibble in the media. The tray type vacuum seeder is made of acrylic sheet and it consists of a vacuum chamber, seed plate with 98 holes to pick the seeds, vacuum pump and necessary control valves. It was observed that the seeder picked and dropped the round shaped seeds like cabbage, cauliflower and knol khol perfectly, i.e., 100% singles. For other vegetable seeds, the metering performance of the seeder was good with singles in the range of 93-97%, doubles between 3-7% and no missing was recorded. It is suggested that these handy and low cost tray type dibbler and tray type vacuum seeder are very much useful for small vegetable nursery growers.
Rathinakumari, A C; Kumaran, G S; Mandhar, S C

Section of Agricultural Engineering, Indian Institute of Horticultural Research, Bangalore, Karnataka 560 089, India.

Key words: cabbages, cauliflowers, containers, design, drilling, drills, equipment performance, mechanization, performance tests, vegetable growing, vegetables, work capacity

Journal of Applied Horticulture, 2005, volume 7, issue 1, pages 49-51.

Abstract: A tray type dibbler with capacity of 100 portrays/h and a vacuum seeder with capacity of 50 portrays/h were designed and developed. The tray type dibbler is made out of wooden board with 98 nylon pegs to dibble in the media. The tray type vacuum seeder is made of acrylic sheet and it consists of a vacuum chamber, seed plate with 98 holes to pick the seeds, vacuum pump and necessary control valves. It was observed that the seeder picked and dropped the round shaped seeds like cabbage, cauliflower and knol khol perfectly, i.e., 100% singles. For other vegetable seeds, the metering performance of the seeder was good with singles in the range of 93-97%, doubles between 3-7% and no missing was recorded. It is suggested that these handy and low cost tray type dibbler and tray type vacuum seeder are very much useful for small vegetable nursery growers.
Rajan, S; Yadava, L P; Ram Kumar; Saxena, S K

Central Institute for Subtropical Horticulture, Rehmankhera, PO Kakori, Lucknow - 227 107, India.

Key words: crop quality, fruits, genetic variation, guavas, heritability, phenotypic variation, seed characteristics, seed weight, seeds, selection criteria

Journal of Applied Horticulture, 2005, volume 7, issue 1, pages 52-54.

Abstract: A total of 68 guava accessions were studied to determine the genetic variability and heritability for fruit weight and associated seed characteristics. Data were recorded for fruit weight, number of seeds per fruit, seed weight per fruit, 100-seed weight, number of seeds per 100 g fruit and seed content. High genotypic (GCV) and phenotypic (PCV) coefficients of variation were observed for all the traits. However, GCV was maximum for pulp:seed weight ratio followed by number of seeds per fruit, 100-seed weight and number of seeds per 100 g fruit. The estimates of PCV ranged from 33.85 (average fruit weight) to 609.75% (pulp:seed weight ratio). The number of seeds per 100 g fruit, number of seeds per fruit and 100-seed weight also exhibited high levels of PCV. The estimates of heritability in the broad sense ranged from 0.558 (seed content) to 0.843 (pulp:seed weight ratio) suggesting that all the characters had high magnitude of heritability. The estimate of genetic advance as percent of mean ranged from 43.76
Rajan, S; Yadava, L P; Ram Kumar; Saxena, S K

Central Institute for Subtropical Horticulture, Rehmankhera, PO Kakori, Lucknow - 227 107, India.

Key words: crop quality, fruits, genetic variation, guavas, heritability, phenotypic variation, seed characteristics, seed weight, seeds, selection criteria

Journal of Applied Horticulture, 2005, volume 7, issue 1, pages 52-54.

Abstract: A total of 68 guava accessions were studied to determine the genetic variability and heritability for fruit weight and associated seed characteristics. Data were recorded for fruit weight, number of seeds per fruit, seed weight per fruit, 100-seed weight, number of seeds per 100 g fruit and seed content. High genotypic (GCV) and phenotypic (PCV) coefficients of variation were observed for all the traits. However, GCV was maximum for pulp:seed weight ratio followed by number of seeds per fruit, 100-seed weight and number of seeds per 100 g fruit. The estimates of PCV ranged from 33.85 (average fruit weight) to 609.75% (pulp:seed weight ratio). The number of seeds per 100 g fruit, number of seeds per fruit and 100-seed weight also exhibited high levels of PCV. The estimates of heritability in the broad sense ranged from 0.558 (seed content) to 0.843 (pulp:seed weight ratio) suggesting that all the characters had high magnitude of heritability. The estimate of genetic advance as percent of mean ranged from 43.76
Hardevinder Singh; Cheema, D S

Department of Vegetable Crops, Punjab Agricultural University, Ludhiana - 141 004, India.

Key words: characteristics, firmness, genetic variation, heat stress, heritability, lycopene, pericarp, phenotypic variation, tomatoes

Journal of Applied Horticulture, 2005, volume 7, issue 1, pages 55-57.

Abstract: Studies were conducted on 15 advance generation breeding lines of tomato, including 4 control cultivars, to study the variation and heritability of quality characteristics in tomato raised under normal and high temperature conditions (November and February, respectively). Data were recorded for total soluble solids (TSS), pericarp thickness, fruit firmness, acidity, lycopene content and dry matter content. There were significant differences among the genotypes under normal conditions, whereas differences were not significant under high temperature conditions. The population mean was higher during November than February planting for all the characters except acid content and TSS. In general, the phenotypic coefficients of variation were higher than genotypic coefficients of variation indicating that the genotypic effect is lessened under the influence of the given environment. Heritability estimates (in the broad sense) were high for all the characters for November planting except for lycopene content.
Hardevinder Singh; Cheema, D S

Department of Vegetable Crops, Punjab Agricultural University, Ludhiana - 141 004, India.

Key words: characteristics, firmness, genetic variation, heat stress, heritability, lycopene, pericarp, phenotypic variation, tomatoes

Journal of Applied Horticulture, 2005, volume 7, issue 1, pages 55-57.

Abstract: Studies were conducted on 15 advance generation breeding lines of tomato, including 4 control cultivars, to study the variation and heritability of quality characteristics in tomato raised under normal and high temperature conditions (November and February, respectively). Data were recorded for total soluble solids (TSS), pericarp thickness, fruit firmness, acidity, lycopene content and dry matter content. There were significant differences among the genotypes under normal conditions, whereas differences were not significant under high temperature conditions. The population mean was higher during November than February planting for all the characters except acid content and TSS. In general, the phenotypic coefficients of variation were higher than genotypic coefficients of variation indicating that the genotypic effect is lessened under the influence of the given environment. Heritability estimates (in the broad sense) were high for all the characters for November planting except for lycopene content.
Pant, T; Bhatt, R P; Bhoj, A S; Kumar, N

Defence Agricultural Research Laboratory, Pithoragarh - 262 501, India.

Key words: crop yield, cucumbers, fruits, hydroponics, leaf area index, photosynthesis, plant density, protected cultivation, soilless culture, transpiration

Journal of Applied Horticulture, 2005, volume 7, issue 1, pages 58-60.

Abstract: A greenhouse experiment was conducted to identify the suitable plant density (2, 4, 6, 8 and 10 plants per m2) for growing cucumber (cv. Green Long) in recirculating hydroponic system of cultivation. An increase in plant density from 2 to 6 plants per m2 significantly increased yield. A declining trend in yield and fruit number was observed at more than 6 plants per m2. The leaf area index and photosynthetic rate in different treatments were also recorded. The photosynthetic and transpiration rates were maximum when plant density was maintained at 6 plants per m2.
Pant, T; Bhatt, R P; Bhoj, A S; Kumar, N

Defence Agricultural Research Laboratory, Pithoragarh - 262 501, India.

Key words: crop yield, cucumbers, fruits, hydroponics, leaf area index, photosynthesis, plant density, protected cultivation, soilless culture, transpiration

Journal of Applied Horticulture, 2005, volume 7, issue 1, pages 58-60.

Abstract: A greenhouse experiment was conducted to identify the suitable plant density (2, 4, 6, 8 and 10 plants per m2) for growing cucumber (cv. Green Long) in recirculating hydroponic system of cultivation. An increase in plant density from 2 to 6 plants per m2 significantly increased yield. A declining trend in yield and fruit number was observed at more than 6 plants per m2. The leaf area index and photosynthetic rate in different treatments were also recorded. The photosynthetic and transpiration rates were maximum when plant density was maintained at 6 plants per m2.
Satoh, S; Nukui, H; Inokuma, T

Laboratory of Environmental Biotechnology, Graduate School of Agricultural Sciences, Tohoku University, Tsutsumidori-amamiyamachi 1-1, Sendai 981-8555, Japan.

Key words: carnations, cut flowers, methodology, preservatives, sucrose, vase life

Journal of Applied Horticulture, 2005, volume 7, issue 1, pages 8-10.

Abstract: An attempt was made to determine the vase life of spray type carnation flowers by observing the number of open flowers, i.e. the percentage of open flowers to the total number of initial flower buds, and to evaluate the efficacy of this method. The vase life determined by this method was similar to that determined by measuring ethylene production and observing senescence symptoms of carnation flowers. The method effectively evaluated the action of preservatives, sucrose and 1,1-dimethyl-4-(phenylsulfonyl)semicarbazide in carnation flowers. The results indicated that this method can be used as an alternative method for the determination of the vase life of carnation flowers, especially those of the spray type.
Satoh, S; Nukui, H; Inokuma, T

Laboratory of Environmental Biotechnology, Graduate School of Agricultural Sciences, Tohoku University, Tsutsumidori-amamiyamachi 1-1, Sendai 981-8555, Japan.

Key words: carnations, cut flowers, methodology, preservatives, sucrose, vase life

Journal of Applied Horticulture, 2005, volume 7, issue 1, pages 8-10.

Abstract: An attempt was made to determine the vase life of spray type carnation flowers by observing the number of open flowers, i.e. the percentage of open flowers to the total number of initial flower buds, and to evaluate the efficacy of this method. The vase life determined by this method was similar to that determined by measuring ethylene production and observing senescence symptoms of carnation flowers. The method effectively evaluated the action of preservatives, sucrose and 1,1-dimethyl-4-(phenylsulfonyl)semicarbazide in carnation flowers. The results indicated that this method can be used as an alternative method for the determination of the vase life of carnation flowers, especially those of the spray type.
Puchooa, D

Faculty of Agriculture, University of Mauritius, Reduit, Mauritius.

Key words: callus, gene expression, genes, genetic engineering, genetic transformation, in vitro culture, in vitro regeneration, leaves, tissue culture, transgenic plants

Journal of Applied Horticulture, 2004, volume 6, issue 2, pages 11-15.

Abstract: Green-fluorescent protein (GFP) gene expression was observed in tissues of litchi (Litchi chinensis) after transformation using Agrobacterium. In vitro grown leaf tissues were used for transformation. After four weeks in culture, expression of GFP was apparent when the regenerated callus and the leaves were observed under fluorescence microscope fitted with a blue exciter filter, a blue dichroic mirror and a barrier filter. Although no transformed litchi plantlets were regenerated, screening for GFP gene expression may prove useful to improve transformation efficiency and to facilitate detection of transformed litchi plants.

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Journal of Applied Horticulture