Metabolite profile of ethanol extract of Curcuma domestica Val. variety Turina-1
1Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University, Bali, Indonesia. 2Present address: Faculty of Agricultural Technology, Udayana University, Bukit Jimbaran, South Kuta, Badung, Bali (80362), Indonesia.
DOI: https://doi.org/10.37855/jah.2021.v23i01.02
Key words: LC-MS, profile metabolite, ethanolic extracts, Curcuma domestica Val, Turina-1
Abstract: The metabolite profile of Curcuma domestica Val. variety Turina-1, one of the superior varieties of turmeric was studied by analysing ethanol extract of the variety. The samples for this research were obtained from BPPT Bogor-Indonesia and were extracted using ethanol (96 %) and then analyzed using UPLC-QToF-MS/MS System (Waters), mass spectrometry: XEVO-G2QTOF (Waters), in ESI positive resolution mode, using gradient method with mobile phase: water, formic acid and acetonitrile. The study revealed 13 metabolites viz., Demethoxycurcumin-2 (48.23 %), ?-Turmerone (19.623 %), Curcumin (18.550 %), Bisdemethoxycurcumin-3 (9.064 %), Curcumin-1, (1.706 %), and other compounds with amount less than 1 % (Kaempferol 3-O-glucosyl-rhamnosyl-galactoside, Demethoxycurcumin, ar-Turmerone Bisdemethoxycurcumin, a-Terpinolene, L-Tyrosine and L-Alanine, L-serine). Based on this research, the main metabolite in the ethanol extract of Turina-1 having the potential as antioxidants is the curcuminoids.
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