Assessing genetic diversity among Bhagwa like pomegranate (Punica granatum L.) genotypes by Inter Simple Sequence Repeat (ISSR) Markers
Department of Horticulture, Mahatma Phule Krishi Vidyapeeth, Rahuri, Maharashtra, India. Department of Horticulture, Mahatma Phule Krishi Vidyapeeth,, Rahuri, Maharashtra, India. State Level Biotechnology Centre, Mahatma Phule Krishi Vidyapeeth,, Rahuri, Maharashtra, India.
DOI: https://doi.org/10.37855/jah.2018.v20i01.15
Key words: Punica granatum, pomegranate, Bhagwa, ISSR, genetic diversity, molecular characterization
Abstract: In the present study, molecular characterization of pomegranate cultivar ‘Bhagwa’ and ‘Bhagwa’ like genotypes available in Maharashtra of India was undertaken along with two other cultivars, Phule Arakta and Mridula to assess their authenticity and diversity. All the 14 ISSR primers used were found polymorphic producing a total of 171 reproducible amplicons with an average of 12.5 amplicons per primer, out of which 144 amplicons (90.28 %) were polymorphic. The similarity coefficient between the genotypes varied from 0.00 to 0.94. Maximum similarity coefficient (0.94) was observed among four different genotypic combinations and high similarity coefficient (? 0.90) was observed among 18 different genotype combinations indicating that these genotypes are least divergent from each other. The UPGMA based cluster analysis using dice similarity coefficient grouped 19 pomegranate genotypes into two major clusters. The closely related genotypes of ‘Bhagwa’ cultivar of pomegranate could be clearly differentiated from cultivars Phule Arakta and Mridula.
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