Exploiting double haploidy in cauliflower (Brassica oleracea var. botrytis L.) for crop improvement

Abstract: A study was conducted to augment a viable protocol for the development of double haploid system in cauliflower, using in-house genotypes, by anther culture. A total of 13,680 anthers were cultured on different media combinations across nine genotypes. Genotype-to-(segregating) genotype variations in anther culture to plant regeneration were observed, chiefly, segregating genotype-9, had highest frequency of regeneration (26.43%) whereas, segregating genotype-2, had the lowest frequency (0.2%). Two segregating genotypes remained unresponsive. Anther cultured on modified media showed highest embryogenic callus induction potential as compared to other media compositions. Regeneration media, RM1 and RM2, showed good differentiation and regeneration potential. In total, 711 haploid / double haploid plants were developed across seven genotypes, with an average regeneration efficiency of 7.6%. This simple protocol reported here, can be used for DH line development in cauliflower, through anther culture, resulting in embryogenic callus induction and plant regeneration. Further, protocol developed and discussed in the paper, will help to exploit DH technology in cauliflower for rapid inbred line development, and to speed-up cauliflower breeding for faster cultivar and better hybrid development.