Media standardization for pre-hardening and hardening of in vitro regenerated plantlets of Gerbera cultivars

Abstract: Experiment was carried out to standardize media for pre-hardening and hardening of in vitro regenerated plantlets taking two cultivars of gerbera namely Red Star and Jallisse. During hardening, soil, sand, FYM and coco peat mixture with a proportion of 1:1:1:1, 1:1:1:2,1:1:2:1, 1:2:1:1, ½:1:1:1 , ½:1:1:2, ½:1:2:1, ½:2:1:1, respectively and control (only soil) were tried for transplanting the regenerated plants. Among these, soil: sand: FYM: coco peat in ½:1:1:2, ½ :2:1:1 and ½:1:1:1 proportion for cv. Jallisse and ½:1:1:2 proportion for cv. Red star were found to be most promising combinations for 100% survival of regenerated plants in both the cultivars.