Assessment of genetic diversity in cucumber varieties using RAPD markers
Department of Biotechnology and Plant Breeding, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. 2Genetics and Agricultural Biotechnology Institute of Tabarestan, Agricultural Sciences and Natural Resources University, Sari, Iran.
DOI: https://doi.org/10.37855/jah.2016.v18i01.14
Key words: Cucumber, Genetic diversity, Cluster analysis, RAPD markers
Abstract: Cucumber (Cucumis sativus L.) is one of the most economically important plants in many countries of the world. The identification of cultivars is extremely important both for cultivation and breeding of crop plants. There is little known about the genetic relationships between cucumber genotypes in Iran. The genetic diversity and the relationships among 20 cucumber varieties were evaluated by RAPD markers. A total of 155 bands were generated with15 RAPD primers, out of which 114 bands were polymorphic (73 %). The mean polymorphism index content (PIC) was 0.24. Considerable levels of polymorphism were observed within cucumber varieties. Cluster analysis based on Jaccard similarity coefficients grouped varieties into three main clusters. The data obtained from this study can be used to select suitable parents in hybridization breeding programs in cucumber.
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