Spectrophotometric determination of total alkaloids in some Iranian medicinal plants
Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
DOI: https://doi.org/10.37855/jah.2010.v12i01.15
Key words: BCG, total alkaloids, medicinal plants, determination
Abstract: A simple spectrophotometric method based on the reaction with Bromocresol Green (BCG) was developed for determination of total alkaloids in medicinal plants. A yellow complex forms and is easily extractable by chloroform at pH 4.7. The absorbance of the complex obeys Bear 's law over the concentration range of 4-13 ug atropine per mL of chloroform. This procedure can be carried out in the presence of other compounds without interference.
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