Abstract: Nineteen X. campestris pv. mangiferaeindicae strains collected from different ecogeographical areas/mango genotypes of India were studied to confirm the existence of variability in the strains on the basis of their pathogenicity on genotypes (9), reaction towards antibiotics (9) and growth on culture media (5). Study revealed the existence of variability in Xcmi strains as exhibited by their differential reaction. The similarity in Xcmi strains was observed by hierarchical cluster analysis. Clustering pattern on these three detection methods indicated that the grouping of strains is not entirely based on their geographical distribution as the strains from northern and southern parts of India falls in a single cluster. However, the strains collected from Bihar exhibited more similarity with each other and clustered in one or nearby cluster in all the detection methods used.