ISSR, anthocyanin content and antioxidant activity analyses to characterize strawberry genotypes
Atlantic Cool Climate Crop Research Centre, Agriculture and Agri-Food Canada, P.O. Box 39088, 308 Brookfield Road, St. John's, NL A1E 5Y7, Canada. Institut Polytechnique LaSalle Beauvais, Rue Pierre Waguet, BP 30313, F-60026 Beauvais Cedex, France
DOI: https://doi.org/10.37855/jah.2009.v11i02.17
Key words: Anthocyanin content, antioxidant activity, Fragaria x ananassa Duch., ISSR markers, strawberry
Abstract: Data on molecular markers, anthocyanin contents and antioxidant activities are increasingly used in breeding programs of many horticultural crops. Inter simple sequence repeat (ISSR) analysis, anthocyanin contents and antioxidant activities were used to characterize 10 strawberry (Fragaria x ananassa Duch.) cultivars and nine breeding lines. Fifteen primers generated 240 polymorphic ISSR-PCR bands. Cluster analysis by the unweighted pair-group method with arithmetic averages (UPGMA) revealed a substantial degree of genetic similarity among the genotypes ranging from 45% to 73% that were in agreement with the principal coordinate (PCO) analysis. Wide genetic diversity was observed among the strawberry genotypes for anthocyanin contents and antioxidant activities. The ISSR analysis together with data for antioxidant activities and anthocyanin contents in strawberries could be used for germplasm management and more efficient choices of parents in current strawberry breeding programs.
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