Effect of growth regulators on in vitro plant regeneration of female papaya using axillary bud as an explant
Department of Biotechnology and Molecular Biology, CCS Haryana Agricultural University, Hisar. 125004, India.
DOI: https://doi.org/10.37855/jah.2009.v11i01.09
Key words: Auxins, axillary bud, benzylaminopurine (BAP), cytokinins, indole acetic acid (IAA), Carica papaya L., a-naphthalene acetic acid (NAA), proliferation, silver nitrate (AgNO3)
Abstract: A study was carried out on mature female papaya (Carica papaya L.) plant of Selection 1 cultivar by using axillary bud as an explant and media supplementation with the main aim to assess the effect of growth regulators (auxins, cytokinins) and silver nitrate on in vitro regeneration of female papaya plant. Total of 28 media were used for shoot regeneration while for root regeneration total of eight media were tested supplemented with different growth hormones. Based on the results of this study, for shoot proliferation, MS basal medium supplemented with BAP (1.0 mg L-1) and BAP (2.0 mg L-1) + NAA (0.1 mg L-1) was found to give the best results while MS medium supplemented with IBA (2.0 mg L-1) gave best rooting percentage. Besides, auxins and cytokinins, effect of silver nitrate (AgNO3) on plant regeneration from axillary buds taken from mature female papaya plant was also carried out.
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