A simple and rapid extraction method to determine osmolar concentration of soluble carbohydrates from rose petals
National Institute of Floricultural Science, Fujimoto, Tsukuba, Ibaraki 305-8519, Japan; Tokyo University of Agriculture, Funako, Atsugi, Kanagawa 243-0034, Japan.
DOI: https://doi.org/10.37855/jah.2008.v10i02.23
Key words: Centrifugal filter device, extraction method, microwave heating, osmotic pressure, petal, rose, soluble carbohydrates.
Abstract: To establish a simple and rapid extraction method for soluble carbohydrate for determination of osmolar concentration in petals by HPLC analysis, a method using a centrifugal filter device with microwave heating was developed. Rose 'Sonia' petals were placed in a centrifugal filter device and heated in a microwave oven to boiling. The centrifugal filter device was centrifuged with the petals at 12,000 g for 10 min. The resulting leached solution was subjected to HPLC analysis. No significant difference in soluble carbohydrate composition was observed between the solution obtained from this method and that obtained from a conventional extraction method in which tissues are homogenized using hot ethanol solution. Changes in soluble carbohydrate concentration with flower opening in 'Rote Rose' roses were investigated using the new method. The osmolar concentrations of glucose and fructose in the petals increased during flower opening. This increase was roughly comparable to the increase in osmotic pressure in the petals. The results suggest that the method using the centrifugal filter device with microwave heating is a simple and rapid way to determine osmolar concentration of soluble carbohydrates of rose petals.
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