Induction of callus and plant regeneration in Coleus forskohlii Briq.
M.T. Asamenew and P. Narayanaswamy
Ethiopian Agricultural Research Organisation, P.O. Box 2003, Addis Ababa, Ethiopia.
DOI: https://doi.org/10.37855/jah.2000.v02i01.07
Key words: adventitious shoots, benzyladenine, callus, culture media, IAA, in vitro culture, in vitro regeneration, kinetin, micropropagation, plant growth regulators, shoot tip culture, tissue culture
Abstract: Callus cultures were initiated from the shoot tip explants of aseptically grown C. forskohlii. A rapid initiation and proliferation of callus was obtained in MS basal medium containing 1.0 mg IAA/l and 1.5 BAP [benzyladenine] mg/l. Adventitious shoots (17.33) were obtained from compact greenish callus on passage to MS basal medium containing various concentrations and combination of IAA and kinetin. But, the best response was in the medium containing 1.0 mg IAA/l and 2.0 mg kinetin/l. On further subculturing of individual shoots onto hormone-free MS medium, shoots developed into normal plantlets.
