Micropropagation of Parthenocissus quinquefolia (L.) from seedling explants
Yun Li, Daling Feng, Zhenyuan Sun and Chen Niu
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China. Current addres College of Life Science, Hebei Agricultural University, Baoding, 071001, China. Research Institute of Forestry,
Abstract: Parthenocissus quinquefolia L. was successfully micropropagated through axillary bud multiplication from seedling explants. Shoot tips were isolated from seedlings and cultured on B5 medium supplemented with 1.33-2.22 uM benzylaminopurine (BA) and 0.107 uM a-naphthaleneacetic acid (NAA) to induce axillary buds. The sprouted axillary buds formed multiple shoots when cultured on B5 medium supplemented with 2.22 uM BA and 0.246-0.49 uM indole-3-butyric acid (IBA). The elongated shoots rooted in B5 medium containing 0.49 uM IBA and they survived from acclimatizing in soil to grow into healthy plants.
