New report of isolates of Lasiodiplodia spp. from the coastal region of Odisha, India causing crown rot disease in Banana
Sarmistha Sahoo1,3, Sushree Suparna Mahapatra1*, Manasranjan Rout1 and Bhagyashree Khamari2
1Department of Plant Pathology, Institute of Agricultural Sciences, Siksha ‘o’ Anusandhan Deemed to be University, Bhubaneswar, 751003, India. 2College of Horticulture, Odisha University of Agriculture and Technology, Chiplima, Sambalpur, 768025, India. 3Department of Plant Pathology, School of Agriculture, GIET University, Odisha, Gunupur. Rayagada, Odisha. India. Corresponding e-mail: sushreesuparnamahapatra@soa.ac.in
Abstract: Crown rot disease in banana caused by the fungus Lasiodiplodia brasiliensis and Lasiodiplodia theobromae is one of the major post-harvest diseases causing a massive loss in the economic yield of banana. Isolates of Lasiodiplodia brasiliensis and Lasiodiplodia theobromae were isolated from infected fruits and were characterized based on their morphological and molecular characteristics. The isolates produced mustard seed-like pycnidia with conidia after 15 days after inoculation (DAI) and chlamydospore was observed after 16 DAI. The immature conidia were hyaline, aseptate, thin-walled and cylindrical to sub-ovoid in shape which became brown with central transverse septum at maturity. The isolates were sequenced and submitted in NCBI gene bank and were assigned with accession no. OQ921841 and OR764951. Further, the evolutionary relationships of the pathogen isolates were studied concerning different related genera and species of Lasiodiplodia.
