Unripe bael pulp pectin extraction and characterization using the microwave assisted method

Abstract: In this study, pectin was extracted from unripe bael using a microwave-assisted extraction technique. Optimization was done based on independent and dependent parameters. Pectin yield was the dependent variable in the study, whereas the independent factors were microwave power, time, and solid solvent ratio. Pectin extraction was carried out using different combinations of microwave power (180, 360, 540 watts), time (1, 2, 3 minutes), and the ratio of solid-solvent (1:20, 1:30, 1:40 g/mL). Using Box-Behnken response surface methodology, 17 experimental runs were performed. A microwave power of 360 watts, a time of 3 minutes, and a solid solvent ratio of 1:20 g/mL were the ideal parameters that achieved the maximum pectin yield of 19.8%. The yield of pectin was analyzed using multiple regression statistical analysis. Qualitative and quantitative analysis of pectin yield was studied, which revealed an equivalent weight of 263.15, a methoxyl content of 9.92 percent, anhydrouronic acid content of 123.2%, and a degree of esterification of 45.71%.