Development and standardization of cactus fruit-based tisane

Abstract: Cactus (prickly pear) is a nutrient-rich fruit with medicinal and health benefits, including minerals (iron, calcium, potassium), vitamins, antioxidants, anti-diabetic, and anti-cancer effects. Mint, a versatile herb, enhances flavor. Herbal fruit teas are increasingly popular. Tisanes are popular for its antioxidant properties mainly because of phenols, which leach in water during infusion. Cactus fruits were dried in hot air dryer and the drying temperature of 50°C was optimized based on minimum drying time, maximum total betalain content and maximum total antioxidant activity. Mint leaves were dried using shade drying at a temperature of 30±2°C up to a moisture content of 7%. Formulation of cactus fruit-based tisane was done by using different cactus-mint ratios. Cactus mint ratio was standardized by highest overall acceptability, total antioxidant activity, and colour a* value. Cactus fruit-based tisane infusion was prepared by dipping a tea bag containing 2 g of dried material in 100 mL of hot water (95±2°C) for a steeping time of 2 min. Based on statistical analysis, the best combination of cactus fruit-mint ratio for tisane was optimized as 85:15. Infusion of cactus fruit-based tisane contained 10.92 mg/100g of total betalain content, 0.89 mg/100g of vitamin C, 12.19 mM/100g of total antioxidant activity etc., and the microbial analysis revealed that the total plate count (cfu/g) was in limit and yeast and mould count (cfu/g) was zero.