Journal Of Applied Horticulture ISSN: 0972-1045


P. Kaur, N. Singh and D. Mukherjee

Laboratory of Plant Physiology and Biochemistry, Kurukshetra University, Kurukshetra-136119, Haryana, India.

Key words: 5-Sulfosalicylic acid, 6-benzylaminopurine, ascorbate peroxidase, catalase, ethanol, guaiacol peroxidase, malondialdehyde, membrane stability index, protein, senescence, sucrose, superoxide dismutase and vase life

Journal of Applied Horticulture, 2015, volume 17, issue 1, pages 31-39.

Abstract: This investigation was carried out to assess not only the efficacy of ethanol (EtOH) with that of sucrose (both are the product of plant metabolism) but also with 6-benzylaminopurine (6-BAP) and 5-sulfosalicylic acid (5-SSA) which are well known plant growth regulators (PGRs) to minimize the decline in certain antioxidant enzymes in the cut flowers of Calendua officinalis L. and Salvia splendens Sellow ex J. A. Schultes. An effect of sucrose was also studied when it was present either alone or in combination with other chemicals (EtOH/6-BAP/5-SSA) in the vase solution. Control cut C. officinalis flowers looked fresh for 1-day, while flowers in sucrose solution lasted for about 2-day whereas other chemicals could extend the vase life from 4 to 7 days. The order of effectiveness of applied chemicals was EtOH + sucrose (Suc) > 5-SSA + Suc > EtOH/5-SSA > 6-BAP > 6-BAP + Suc > Suc in C. officinalis whereas it was EtOH/EtOH + Suc > 5-SSA > 5-SSA + Suc > 6-BAP + Suc > 6-BAP > Suc in S. splendens. In both the plants, petals of untreated flowers exhibited a gradual reduction in activities of ascorbate peroxidase (APX), catalase (CAT), superoxide dismutase (SOD); and protein levels and membrane stability index (MSI) values whereas malondialdehyde (MDA) level and guaiacol peroxidase (GPOX) activity registered increment. However, individual treatment of metabolites like ethanol and sucrose, plant growth regulators like 6-BAP and 5-SSA were able to reduce not only protein content but also activities of APX, CAT and SOD. The combined effect of EtOH + Suc was more effective than 6-BAP + Suc or 5-SSA + Suc.

Journal of Applied Horticulture