Abstract: Experiment was conducted in vitro to see the different physical properties and transmission of papaya ring spot virus with different aphid species. The virus was found to be inactivated between temperature 50 to 55°C and between the dilutions of 10^-3 to 10^-4. It remained viable upto 24 hours at temperature 28 to 30°C and 5 days at 6 to 8°C temperature. The virus was transmissible by five aphid species Aphis gossypii (Glover), Aphis craccivora (Koch), Acyrthosiphonpisum (Buczacki S. and Harris K.), Dactynotus carthami (Hille Ris Lambers), Aphis nerii (Boyer de Fonscolombe) in non persistent manner.