Division of Horticulture, University of Agricultural Sciences, Bangalore - 560 065, India.
Key words: benzyladenine, carbendazim, chlorothalonil, cultivars, disinfectants, explants, IAA, in vitro culture, in vitro regeneration, micropropagation, ornamental plants, plant growth regulators, rooting, roots, shoots, tissue culture, tissue cultures, varietal r
Journal of Applied Horticulture, 2001, volume 3, issue 2, pages 82-84.
Abstract: Terminal or axillary stem scale sections from P. tuberosa cultivars Shringar (single type) and Suvasini (double type) were disinfected with 1000 ppm Bavistin [carbendazim], 1000 ppm Kavach [chlorothalonil], and 500 ppm Cetrimide, singly or in combination, before sterilization with 0.1% HgCl2 for 10-15 minutes. The explants were cultured in Murashige and Skoog's (MS) medium containing 3% sucrose and 0.25% phytagel, and autoclaved at 121 degrees C for 15 minutes. The shoot tips from sprouted explants were transferred into a medium containing 2 or 4 mg BAP [benzyladenine]/litre singly or in combination with 0.1 mg IAA/litre. The regenerated shoots were transferred into 1/2 MS medium containing 0.5 or 1.0 mg IBA, 0.5 mg IAA, or 0.25 mg IAA + 0.25 mg IBA/litre. Treatment with Bavistin + Kavach + Cetrimide overnight followed by treatment with HgCl2 for 15 minutes resulted in the highest percentage of axenic cultures using axillary (23.3-26.6%) and terminal (30.0%) scale sections. Cytokinin induced multiple shoot fo
