Journal Of Applied Horticulture ISSN: 0972-1045


R. Rekha, Pallavi Mandave and Neelambika Meti

Department of Plant Biotechnology, Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth Deemed University, Pune- 4110046, Maharashtra, India.

Key words: Axillary shoot proliferation, strawberry, runner tips, TDZ, daughter runners

Journal of Applied Horticulture, 2012, volume 14, issue 1, pages 71-73.

Abstract: A protocol for micropropagation of strawberry cv. Sweet Charlie was standardized through axillary shoot proliferation from runner tips. Medium supplemented with TDZ (1 mg/L) alone was favourable for the induction of multiple shoots and daughter runners from runner tips. Such shoots were successfully multiplied for four times on MS incorporated with 0.5mg/L each of BAP, IBA and 1.0 mg/L of GA3. Rooting of subcultured shoots was achieved on MS medium containing 0.5 mg/L of kinetin alone and along with 0.5 mg/L of IBA. Ex agar plants were harvested regularly after three weeks of growth period for their acclimatization in both cocopeat and soil. The survival rate of tissue cultured plants was 85%.

Journal of Applied Horticulture