College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China. 1 Current addres College of Life Science, Hebei Agricultural University, Baoding, 071001, China. 2Research Institute of Forestry,
Key words: Parthenocissus quinquefolia, axillary buds, micropropagation, B5 medium, benzylaminopurine (BA), indole-3-butyric acid (IBA), indole-3-acetic acid (IAA), a-naphthaleneacetic acid (NAA)
Journal of Applied Horticulture, 2006, volume 8, issue 2, pages 151-154.
Abstract: Parthenocissus quinquefolia L. was successfully micropropagated through axillary bud multiplication from seedling explants. Shoot tips were isolated from seedlings and cultured on B5 medium supplemented with 1.33-2.22 uM benzylaminopurine (BA) and 0.107 uM a-naphthaleneacetic acid (NAA) to induce axillary buds. The sprouted axillary buds formed multiple shoots when cultured on B5 medium supplemented with 2.22 uM BA and 0.246-0.49 uM indole-3-butyric acid (IBA). The elongated shoots rooted in B5 medium containing 0.49 uM IBA and they survived from acclimatizing in soil to grow into healthy plants.
