Journal Of Applied Horticulture ISSN: 0972-1045


M. Bejoy, M. Dan, N.P. Anish, Githa Ann George and B.J. Radhika

Plant Genetic Resource Division, Tropical Botanic Garden and Research Institute, Palode, Trivandrum 695 562, Kerala, India.

Key words: Amomum hypoleucum, micropropagation, tissue culture, Zingiberaceae

Journal of Applied Horticulture, 2010, volume 12, issue 1, pages 46-49.

Abstract: An efficient and repeatable micropropagation protocol has been established for Amomum hypoleucum, a lesser known threatened medicinal plant of the family Zingiberaceae. Eighty percent of the rhizome nodes from greenhouse grown plants, cultured on MS medium supplemented with 1 mg L-1 BA and 0.5 mg L-1 IAA, showed axillary bud break in 8-10 days. Multiple shoots proliferated from such shoot explants when transplanted to medium with 3 mg L-1 BA and 1 mg L-1 TDZ. An average of 9.2 shoots could be recovered in two months and about 65-70% of the shoots showed simultaneous rooting. Isolated shoots were also rooted in medium fortified with 0.5 mg L-1 NAA. Plantlets, transferred to the field after acclimatization in greenhouse conditions, showed 85% survival.

Journal of Applied Horticulture