Journal Of Applied Horticulture ISSN: 0972-1045


S.N. Saxena, S Janjharia,A.K. Sharma, R. Sharma and G. Singh

Plant Biotechnology Centre, Rajasthan Agricultural University, Bikaner-334006

Key words: Capparis deciduas, shoot tip culture, plant growth regulators, micropropagation, 6-benzyl amino purine, naphthaline acetic acid, indole butyric acid, arid horticulture.

Journal of Applied Horticulture, 2007, volume 9, issue 2, pages 153-156.

Abstract: Capparis deciduas is an important constituent of desert ecosystem, however, due to population pressure on the land their stands are reducing at an alarming rate. Establishment through root suckers and seeds being very slow and cumbersome, remains the major bottleneck for their reestablishment in the area. Thus, realizing the constraint, a rapid and efficient micropropagation protocol for multiple shoot regeneration employing shoot tip explant was developed. The protocol is more efficient and reproducible than reported earlier. Maximum number of explants (100%) responded on Murashige and Skoog (MS) medium supplemented with 6-benzyl amino purine (BAP) 7 mg L-1 and naphthaline acetic acid (NAA) 0.1 mg L-1 while number of shoots per explant were maximum (8.5) on alone BAP 7 mg L-1. Regenerated shoots could be rooted best on MS medium supplemented with indole butyric acid (IBA) 1 mg L-1. Rooted shoots could be hardened and transplanted in to the field.

Journal of Applied Horticulture