Journal Of Applied Horticulture ISSN: 0972-1045


H.M. Mahadeva Swamy, S.N. Nagesha, Prakash M. Navale, T.K.S. Gowda, R. Asokan and Riaz Mahmood

Department of Biotechnology, University of Agricultural Sciences, GKVK, Bangalore 560 065

Key words: Synthetic crylF gene, Agrobacterium tumefaciens, cabbage, transformation, regeneration, kanamycin

Journal of Applied Horticulture, 2013, volume 15, issue 1, pages 3-9.

Abstract: Insect-resistant crops have been one of the major successes of applying plant genetic engineering technology to agriculture. There is an urgent need for improvement in vegetable production, especially in developing countries where the economic, health and environmental benefits of bioengineered vegetables could be of great importance. In this view a synthetic cry1F gene coding for an insecticidal crystal protein of Bacillus thuringiensis (Bt) was transformed to cabbage cultivar 'Hare Krishna' by co-cultivating hypocotyls explants with AAgrobacterium tumefaciens mediated transformation. The transformed plants resistant to kanamycin were regenerated on selection medium. Confirmation of transgene in putatively transformed plants was carried out by using nptII and crylF gene specific primers. Multiple shoot regeneration of hypocotyl and shoot tip explants of cabbage after co-cultivation with Agrobacterium was optimized and medium containing 2 mg/L BAP was observed to be the best for shoot regeneration after co-cultivation. In this study, 45 and 32.5% transformation efficiencies were achieved for hypocotyl and cotyledonary leaf explants, respectively using the optimized procedure.

Journal of Applied Horticulture