Abstract: Evaluation of seventeen genotypes of makoi (Solanum nigrum L.) revealed maximum dry herbage yield in MG-1 (209.07 g) followed by MG-14 (161.73 g). While, for total alkaloid content the genotype MG-13 (0.23 % w/w) recorded highest followed by MG-16 (0.22 %w/w). The genotype MG-14 had maximum total alkaloid yield (12.26 kg ha^-1) followed by MG-13 (11.91 kg ha^-1). Result of genetic studies revealed that phenotypic coefficient of variation was higher than genotypic coefficient of variation for all the traits studied, indicating environmental influence on expression of these characters. Both GCV and PCV were high for total alkaloid yield, moderate to high for remaining traits and low for days to maturity. High heritability coupled with high genetic advance was recorded for leaf area, total alkaloid content and total alkaloid yield indicating the presence of additive gene effects. Hence, selection can be employed for improvement of these characters in makoi. Dry herbage yield per plant was found to be positive and highly significant genotypic association with fresh herbage yield, leaf area, number of leaves, plant spread and plant height and significant with total alkaloid content. The total alkaloid yield had positive and highly significant genotypic correlation with total alkaloid content. The total alkaloid yield had the maximum direct positive effect on dry herbage yield followed by number of leaves and fresh herbage yield. The total alkaloid content and dry herbage yield had the maximum direct effect on total alkaloid yield.