Journal Of Applied Horticulture ISSN: 0972-1045


Neeru Sood; Ranjan Srivastava; Singh, O S; Gosal, S S

Biotechnology Center, Punjab Agricultural University, Ludhiana - 141 004, India.

Key words: bandages, benzyladenine, in vitro culture, in vitro regeneration, kinetin, leaves, micropropagation, plant growth regulators, shoots, strawberries, survival, tissue culture

Journal of Applied Horticulture, 2000, volume 2, issue 2, pages 92-93.

Abstract: The rate of strawberry propagation through conventional technique is quite low and it is difficult to maintain plant material during the summer months. In the present investigation, the protocol for tissue culture propagation has been further improved by using liquid medium with four layers of surgical bandage. Fifteen milliliters of liquid Murashige and Skoog's (MS) medium enriched with BAP [benzyladenine] and Kinetin was used for the study. The proliferating shoots were longer, thicker, and borne with broader leaves in liquid medium. Studies on the survival of such plants were also conducted. The survival rate on various substrates varied from 66.67% in soil to 76.67% in soilrite mix. Seventy percent of plantlets transferred on sand survived well.

Journal of Applied Horticulture