Journal Of Applied Horticulture ISSN: 0972-1045


A.K. Bera, T.R. Maity, A. Samanta, A. Dolai, B. Saha and S. Datta

Mahishadal Raj College, Mahishadal-721 628, West Bengal, India. Haldia Institute of Technology, Haldia-721 657, West Bengal, India.

Key words: Efficient protocol, coconut coir, in vitro corms, Gladiolus, RAPD, genetic fidelity

Journal of Applied Horticulture, 2015, volume 17, issue 3, pages 222-224.

Abstract: An efficient protocol was developed for mass scale in vitro corm production of Gladiolus (cv. ‘White Friendship’) using liquid culture and coconut coir explored as a matrix. The culture was initiated from the basal portion of the innermost leaf of the sprout. The cut surface of responding explant was swelled in MS solid basal media supplemented with 2 mg L-1 NAA. Ten to fifteen shoot buds were observed when the responding explant was transferred in MS media with 2 mg L-1 BAP and 0.2 mg L-1 NAA. The high frequency of in vitro corms were initiated in liquid MS media supplemented with 0.5 mg L-1 NAA and 6% sucrose in coir matrix. The corms with highest mean fresh weight (4570 mg) and diameter (25 mm) developed on periodically replaced media (every three weeks) than unchanged media. Using RAPD profiling, the genetic fidelity of in vitro raised corms were tested.

Journal of Applied Horticulture