Journal Of Applied Horticulture ISSN: 0972-1045


Shekafandeh and M. Ghasemi

Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, Iran.

Key words: Benzyladenin (BA), late-flowering, nodal segments, proliferation, hot water

Journal of Applied Horticulture, 2009, volume 11, issue 2, pages 143-145.

Abstract: In this study, hot-water and cold treatments were used for eradication of explant contamination, and also the effect of plant growth regulators on shoot proliferation was evaluated. The explants were nodal segments of a late flowering almond cultivar 'Sharood 7'. Experiments were carried out in a complete randomized design with 25 replications. All hot-water treatments eliminated fungal contamination. The best hot-water treatment was 50?C in which 88% of explants were both free of contamination and necrosis followed by 76% at 47.5?C and 56% at 45?C. The best proliferation rate obtained in 1.5 mg L-1 BA in combination with 0.1 mg L-1 IBA (5.25 shoots per explant) which was significantly higher than 1 mg L-1 (2.65 shoots per explant). Cold treatments only (2 and 4 days in 4?C) delayed fungal contaminations for 7 days, so it was impossible to assess bacterial contamination.

Journal of Applied Horticulture