Journal Of Applied Horticulture ISSN: 0972-1045


Mohapatra, A; Rout, G R

Plant Tissue Culture Laboratory, Plant Biotechnology Division, Regional Plant Resource Centre, Bhubaneswar - 751 015, Orissa, India.

Key words: auxins, benzyladenine, buds, culture media, cytokinins, IAA, in vitro culture, in vitro regeneration, micropropagation, plant growth regulators, rooting, shoots, sucrose, tissue culture

Journal of Applied Horticulture, 2004, volume 6, issue 2, pages 27-29.

Abstract: Efficient and rapid micropropagation system was developed for Geoderum purpureum [Geodorum purpureum], an important terrestrial orchid, through axillary meristem culture by manipulating cytokinin and auxin. Multiple shoots were induced from axillary meristems cultured on agar-based MS medium supplemented with 2.0-3.0 mg benzyladenine/litre, 0.5-1.0 mg IAA/litre and 3% (w/v) sucrose. Maximum number of shoot buds were obtained with 3.0 mg benzyladenine and 1.0 mg IAA/litre. The rate of shoot multiplication was maintained in subsequent subculture on similar fresh culture medium. Elongated shoots were separated and rooted on half strength basal MS medium supplemented with IAA or IBA and 2% (w/v) sucrose. Maximum percentage of rooting was obtained on medium having 0.5 mg IAA/litre. Plantlets, thus developed were established in soil with 80% survival.

Journal of Applied Horticulture