A. Vitkova, A. Gavrilova, M. Delcheva, A. Trendafilova and M. Todorova
Department of Plant and Fungal Diversity and Resources, Institute of Biodiversity and Ecosystem research, Bulgarian Academy of Sciences, Sofia, Bulgaria, Faculty of Forestry, University of Forestry, Sofia, Bulgaria. Institute of Organic Chemistry with Ce
Key words: Alchemilla achtarowii Pawl., A. jumrukczalica Pawl., A. mollis (Buser.) Rothm., medicinal plants, endemics, ex situ, biological productivity, flavonoids, tannins
Abstract: Guidelines for introduction and ex situ cultivation of species from genus Alchemilla, known by its curative properties for a variety of health disorders are presented for the first time. Subject of the study are the rare and protected Bulgarian species Alchemilla achtarowii Pawl., A. jumrukczalica Pawl. and A. mollis (Buser.) Rothm., which showed high antioxidant activity in our recent research. Transplant material from natural populations grown in two live collections in the regions of Vitosha Mt. (1404 masl) and West Rhodopes Mt. (1500 masl) (Bulgaria) was used. The growth and development rate of the new plants was assessed according to the method of phenological observations. Nine morphometrical indices were studied and the biological productivity of the species was determined in ex situ conditions. The quantities of flavonoids (calculated as % quercetin) and tannins (calculated as % pyrogallol) during the different phenological stages of ex situ plant development were assessed via spectrophotometric methods. Several differences between the species in the two experimental stations were summarized as dependent on the ecological conditions. All results of the study were used to elaborate methodological instruction for successful cultivation of the species in field conditions.