Department of Horticulture, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India.
Key words: plant growth regulators, in vitro culture, in vitro regeneration, roses, micropropagation, gibberellic acid, IBA, NAA, rooting, acclimatization, roots, ornamental woody plants, shoots, gibberellins, tissue culture, ornamental plants, auxins, cytokinins
Journal of Applied Horticulture, 1999, volume 1, issue 2, pages 91-93.
Abstract: A micropropagation method for roses cv. Sonia is presented. Shoot proliferation was best (70.3%) on MS [Murashige and Skoog] medium supplemented with BAP [benzyladenine] at 2 mg/litre + NAA at 0.1 mg/litre + GA3 at 0.01 mg/litre, with a proliferation of >5 microshoots per subculture. Efficient rooting was achieved on half-strength MS medium supplemented with IBA at 0.2 mg/litre + NAA at 0.1 mg/litre. Rooted plantlets were acclimatized for 3 weeks and planted out under field conditions with a survival of over 70%.
