Journal Of Applied Horticulture ISSN: 0972-1045


Yu Zhou and Bal Ram Singh

Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, 285 Old Westport Road, North Dartmouth, MA 02747 USA.

Key words: Anthocyanin, biosynthesis, callus, cranberry, Ericaceae, growth, light, pH, temperature, Vaccinium macrocarpon Ait

Journal of Applied Horticulture, 2007, volume 9, issue 1, pages 17-21.

Abstract: Cranberry callus was successfully induced from cranberry (Vaccinium macrocarpon Ait, Ericaceae) leaves by using Gamborg's B5 medium containing phytohormones at 25oC in the dark. Anthocyanin-producing cranberry callus was obtained only under conditions of continuous light exposure. Red light and UV light exposure of the callus enhanced anthocyanin content by 41.3 and 29.3%, respectively. The light-dependent anthocyanin production in the callus was regulated by temperature. Anthocyanin content in the callus decreased 81.1% at 42oC, 58.9% at 37oC, 47.0% at 30oC, and increased 10.4% at 4oC, compared to the callus maintained at 25oC after 48 hours of incubation at the given temperature. A temperature decrease of 10oC from 25 to 15oC resulted in a critical increase of the anthocyanin production in the callus, irrespective of differences in pH of culture medium. The growth of the callus cultured in medium at pH 7.0 was 6.2-fold higher than in the same medium at standard pH of 5.8.

Journal of Applied Horticulture