Journal Of Applied Horticulture ISSN: 0972-1045


Ang BoonHaw; Chan LaiKeng

School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.

Key words: benzyladenine, culture media, IBA, in vitro culture, in vitro regeneration, micropropagation, plant growth regulators, shoots, tissue culture

Journal of Applied Horticulture, 2003, volume 5, issue 2, pages 65-68.

Abstract: S. acmella [Blainvillea acmella] was successfully micropropagated using axillary buds as explants. The aseptic axillary buds formed multiple shoots within 5 weeks when cultured on MS medium supplemented with 2.0, 4.0, 6.0 and 8.0 mg benzyladenine (BA)/l. The addition of IBA as low as 2 mg/l into the MS medium containing BA had no significant effect on the multiple shoot formation. MS medium supplemented with 0.5 mg BA/l was sufficient for the proliferation of rooted multiple shoots. First subculturing of the in vitro individual shoots in the same proliferation medium could double the formation of multiple shoots.

Micropropagation of Spilanthes acmella L., a bio-insecticide plant, through proliferation of multiple shoots.

Journal of Applied Horticulture