WWW
horticultureresearch

Combined effects of spermine, 8-hydroxyquinoline and sucrose on postharvest physiology and vase life of Dendrobium cv. Sonia 17

Y. Auty, Alka Singh*, Anuradha Thabbannavar, Dipal Bhatt, H.P. Shah and G.D. Patel

*Department of Floriculture & Landscape Architecture, ASPEE College of Horticulture, Navsari Agricultural University, Navsari-396450, Gujarat. Corresponding e-mail: alkaflori@nau.in

DOI: https://doi.org/10.37855/jah.2026.v28i02.73

Key words: Dendrobium, cut spikes, spermine, 8-hydroxyquinoline, sucrose, GA3, lipoxygenase, lipid peroxidation, electrolyte leakage, tepal, senescence, vase life
Abstract:

An investigation was conducted to study the synergistic effect of spermine, 8-hydroxyquinoline and sucrose on postharvest physiology and flower quality of Dendrobium cut spikes. The experiment was arranged in a completely randomised design with 10 vase-solution treatments including a tap-water control, and three replications. The cut spikes were kept under controlled conditions of temperature 24 ± 2 °C, relative humidity 60–70% and photoperiod 8 ± 2 h day-1. Different chemicals, including 8-hydroxyquinoline, sucrose, ?-lipoic acid, citric acid, aluminium sulphate, spermine and GA3 and their combinations were tested as vase solution treatments. Among all the vase solution treatments, spermine (100 mg L-1) + 8-hydroxyquinoline (300 mg L-1) + sucrose (3%) was found to be most effective in maintaining quantitative parameters like water uptake and total soluble sugars in tepal tissue of orchid cut spikes.The same vase solution treatment maintained higher catalase activity while reducing lipoxygenase activity, lipid peroxidation, and electrolyte leakage in tepal cells, thus significantly delaying senescence. The best treatment prolonged vase life up to 29.30 days which is 96% more than the control. Further, qualitative parameters were influenced by vase solution treatments. Also, percentage bud opening per spike and anthocyanin pigment content in tepals of Dendrobium cut spikes held in vase solution treatment comprising of spermine (100 mg L-1) + 8-hydroxyquinoline (300 mg L-1) + sucrose (3%) were significantly improved over control.




Journal of Applied Horticulture