Effect of growth hormones on micropropagation of a farmers’ preferred local cassava (Manihot esculenta Crantz) cultivar from nodal explants in Burkina Faso

W.I.C. Ouédraogo; K. Somé; M. Soro; F. Tiendrébéogo<sup>3; 4</sup>; D. Tiama; A. Sory; R. Nana

ISSN: 0972-1045

Effect of growth hormones on micropropagation of a farmers’ preferred local cassava (Manihot esculenta Crantz) cultivar from nodal explants in Burkina Faso

Journal of Applied Horticulture, 2026, 28(1): 252-256.

Effect of growth hormones on micropropagation of a farmers’ preferred local cassava (Manihot esculenta Crantz) cultivar from nodal explants in Burkina Faso

W.I.C. Ouédraogo¹*, K. Somé², M. Soro³, F. Tiendrébéogo^3,4, D. Tiama², A. Sory¹ and R. Nana¹

¹Equipe Ecophysiologie Végétale, Laboratoire Biosciences, Ecole Doctorale Sciences et Technologies, Université Joseph Ki-Zerbo, Ouagadougou 03, 03 BP 7021, Burkina Faso. ²Laboratoire de Génétique et de Biotechnologies Végétales/ Programme Plantes à Racines et Tubercules, Institut de l’Environnement et de Recherches Agricoles (INERA), 01 BP 476 Ouagadougou 01, Burkina Faso. ³Laboratoire de Virologie et de Biotechnologies Végétales, Institut de l’Environnement et de Recherches Agricoles (INERA), 01 BP 476 Ouagadougou 01, Burkina Faso. ⁴Central and West African Virus Epidemiology (WAVE), Université Felix Houphouêt-Boigny Pôle scientifique et d’innovation, Bingerville, BPV 34 Abidjan - Côte d’Ivoire. Corresponding e-mail: calebouedraogo09@gmail.com

DOI: https://doi.org/10.37855/jah.2026.v28i01.40

Key words: Manihot esculenta, micropropagation, nodal explant, auxin, cytokinin, BAP, NAA, in vitro culture, cassava regeneration

Abstract:

Adopting micropropagation methods to propagate local cassava (Manihot esculenta) cultivars of Burkina Faso could greatly enhance farmers’ access to high-quality cassava cuttings. However, the optimal composition of the growth medium may vary depending on the cassava genotype. This study aimed to assess the individual effects of 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) on in vitro plantlet stem and root growth of the preferred local cassava cultivar ‘BFM260’. To achieve this, nodal explants were inoculated onto five media: one hormone-free control and four media supplemented with NAA or BAP. The experiment was conducted using a completely randomized design (CRD) consisting of five treatments with three replications. Four weeks after inoculation, the hormone-free medium (M0) produced the longest root (60.20 ± 5.32 mm). The plantlets grown on the culture medium supplemented with 0.02 mg L^-1 of NAA (M3) induced a 100% nodal explants regeneration rate, exhibited the longest stem (14 ± 2.84 mm) and produced the highest number of roots (3.6 ± 0.29). This medium was superior for overall plantlet architecture and multiplication potential. However, the high callus rate observed at the base of the plantlets produced on medium M3 requires that the survival rate of these plantlets during acclimatization be evaluated.