Journal of Applied Horticulture. 2000 publ 2001: 2(1): 28-30

In vitro plant regeneration of Citrus aurantifolia through callus culture

Srivastava, R K; Sandhu, A S; Neeru Sood

Department of Horticulture, Punjab Agricultural University, Ludhiana - 141 004, India.

Callus was induced from different explants of in vitro raised seedlings of C. aurantifolia to study the response of explants and medium composition on frequency of callusing and to develop reliable protocol for high frequency of plant regeneration from callus cultures. Shoot tip, epicotyl and hypocotyl were found superior explants for callusing in terms of amount of callusing, days to callus and callus induction frequency. Addition of cytokinin was found indispensable for regeneration and MS medium enriched with BAP [benzyladenine] (5 mg/l) resulted in highest per cent of callus regeneration. Regenerants were rooted in vitro and hardened in plastic pots containing autoclaved soil.