Journal of Applied Horticulture. 1999: 1(2): 91-93

Critical studies on the effect of growth regulators on in vitro shoot proliferation in Rosa x hybrida L. cv. Sonia for micropropagation

Singh, S K; Syamal, M M

Department of Horticulture, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India.

A micropropagation method for roses cv. Sonia is presented. Shoot proliferation was best (70.3%) on MS [Murashige and Skoog] medium supplemented with BAP [benzyladenine] at 2 mg/litre + NAA at 0.1 mg/litre + GA3 at 0.01 mg/litre, with a proliferation of >5 microshoots per subculture. Efficient rooting was achieved on half-strength MS medium supplemented with IBA at 0.2 mg/litre + NAA at 0.1 mg/litre. Rooted plantlets were acclimatized for 3 weeks and planted out under field conditions with a survival of over 70%.