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Micropropagation studies in "single" vs.
"double" types of tuberose (Polianthes tuberosa L.).
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| Krishnamurthy,
K. B.,Mythili, J. B.,Meenakshi Srinivas |
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Division of Horticulture, University of
Agricultural Sciences, Bangalore - 560 065, India.
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| Terminal or
axillary stem scale sections from P. tuberosa cultivars Shringar (single
type) and Suvasini (double type) were disinfected with 1000 ppm
Bavistin [carbendazim], 1000 ppm Kavach [chlorothalonil], and 500 ppm
Cetrimide, singly or in combination, before sterilization with 0.1%
HgCl2 for 10-15 minutes. The explants were cultured in Murashige and
Skoog's (MS) medium containing 3% sucrose and 0.25% phytagel, and
autoclaved at 121 deg C for 15 minutes. The shoot tips from sprouted
explants were transferred into a medium containing 2 or 4 mg BAP
[benzyladenine]/litre singly or in combination with 0.1 mg IAA/litre.
The regenerated shoots were transferred into 1/2 MS medium containing
0.5 or 1.0 mg IBA, 0.5 mg IAA, or 0.25 mg IAA + 0.25 mg IBA/litre.
Treatment with Bavistin + Kavach + Cetrimide overnight followed by
treatment with HgCl2 for 15 minutes resulted in the highest percentage
of axenic cultures using axillary (23.3-26.6%) and terminal (30.0%)
scale sections. Cytokinin induced multiple shoot formation, however, the
addition of auxin increased the regeneration response. A multiple shoot
induction of 100% was obtained with 2 mg BAP + 0.1 mg IAA/litre. Shoot
length increased with the increase in BAP level. A rooting percentage
of 100%, along with the highest average number of roots (16.70 in
Shringar and 15.8 in Suvasini), was recorded for 0.2 mg IAA + 0.25 mg
IBA/litre. The longest roots (1.39 cm in Shringar and 1.33 cm in
Suvasini) was obtained with 0.5 mg IBA/litre. Suvasini exhibited
greater regeneration potential than Shringar. The rooted plantlets were
hardened in polybags containing autoclaved soil, sand, and soilrite
(1:2:1). The hardened plants showed 100% survival in pots. . |
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