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Effect of nodal position and season on in
vitro shoot proliferation in aonla (Emblica officinalis Gaertn.).
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| Maneesh
Mishra,Pathak, R. K |
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N.D. University of Agriculture and
Technology, Kumarganj, Faizabad (UP) - 224 221, India.
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| Emblica
officinalis [Phyllanthus emblica ] nodal shoots excised from the top or
middle portion of the canopy (1st to 10th, 10th to 15th, 15th to 20th,
20th to 25th, or 25th to 30th node) from April to July, August or
November, or December to March were cultured in Murashige and Skoog's
medium containing 0.8% agar, 3% sucrose, 0.4 mg kinetin/litre, and 0.4
mg gibberellic acid/litre at 25 plus or minus 2 deg C and 50-55%
relative humidity. Nodal shoots excised from the 10th to the 15th node
exhibited the greatest bud induction and produced the longest
indeterminate shoots (0.83 cm). The shoots excised from the 1st to the
10th node did not survive due to the inability of the shoots to
withstand the toxic effect of sterilants an antioxidants. The shoots
collected from the 20th to the 30th node showed low bud induction,
probably due to tissue maturity. Bud induction and growth of
indeterminate shoots were most pronounced in explants collected during
August-November. Bud break was not observed in explants collected during
December-March because this period coincided with the dormancy period
in E. officinalis . The highest bud induction rate (76.40%) was
obtained with explants excised from the 10th to the 15th node during
August-November. |
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