|
Effect of surface sterilizing agents on in vitro culture establishment
of guava (Psidium guajava L.). |
| Meghwal, P.
R.,Sharma, H. C.,Singh, S. K. |
|
Division of Fruits and Horticultural Technology, IARI, New Delhi - 110
012, India. |
| A method was
standardized for the quick establishment of aseptic cultures in guava
from mature field-grown stock plants for micropropagation through
enhanced axillary branching technique. The maximum number of aseptic
explants with shoot proliferation was obtained by a combination of
surface sterilizing agents involving hydrogen peroxide (10%), silver
nitrate (0.25%), and mercuric chloride (0.05%) treatment of explants one
by one for five, six, and three minutes, respectively. The problem of
phenolic browning was also minimized to a great extent by leaching of
phenolic compounds due to agitation in antioxidant solution as well as
by proper drying of explant prior to inoculation. |
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