2,4,5-T
induced somatic embryogenesis in papaya (Carica papaya L.). |
Bhattacharya,
J.,Khuspe, S. S. |
Plant Tissue
Culture Division, National Chemical Laboratory, Pune - 411 008, India |
A protocol
for high frequency somatic embryogenesis in C. papaya was developed
using immature zygotic embryo explant of cultivars Honey Dew and CO 2.
Somatic embryos were induced in immature embryos, cultured on Murashige
and Skoog's (MS) basal medium supplemented with 3 mg/litre of 2,4,5-T,
and incubated in the dark for a period of 3-6 weeks. Loosely attached
globular somatic embryos appeared from apical domes within 3-6 weeks of
incubation. The development of somatic embryos was asynchronous, which
passed through globular, heart, and torpedo shape stages. Embryos
continued to proliferate with regular subculture and remained
morphologically competent for up to one year. Maturation of the embryos
was achieved in medium supplemented with ABA [abscisic acid] (0.1
mg/litre). The cotyledonary stage embryos germinated (71.33% in Honey
Dew and 59.33% in CO 2) on phytohormone free MS basal medium.
Regenerated plantlets were established in the greenhouse and hardened
plants were transferred in soil. |
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