|
Induction of callus and plant regeneration in Coleus forskohlii Briq. |
| Asamenew,
M. T.,Narayanaswamy, P. |
|
Ethiopian Agricultural Research Organisation, P.O. Box 2003, Addis
Ababa, Ethiopia. |
| Callus
cultures were initiated from the shoot tip explants of aseptically grown
C. forskohlii . A rapid initiation and proliferation of callus was
obtained in MS basal medium containing 1.0 mg IAA/l and 1.5 BAP
[benzyladenine] mg/l. Adventitious shoots (17.33) were obtained from
compact greenish callus on passage to MS basal medium containing various
concentrations and combination of IAA and kinetin. But, the best
response was in the medium containing 1.0 mg IAA/l and 2.0 mg
kinetin/l. On further subculturing of individual shoots onto
hormone-free MS medium, shoots developed into normal plantlets. |
|
|
|
|
