| Antibiotics
as an aid for in vitro establishment of shoot tips from field-grown
papaya plants of var. 'Pusa Nanha' for mass cloning. |
| Singh, S.
K.,Sharma, H. C.,Singh, S. P. |
| Division of
Fruits and Horticultural Technology, IARI, New Delhi-110 012, India |
| Agitating
shoot tip explants from 14-month-old field-grown plants of pawpaw cv.
Pusa Nanha in an antibiotic solution (Cefotaxime at 50 mg litre-1 +
Gentamycin at 100 mg litre-1 + Streptomycin at 50 mg litre-1) prior to
culture on establishment medium significantly reduced problems of
systemic bacterial contamination present in the tissue. On average 4-5
shootlets were proliferated during 6 subcultures on MS basal medium
supplemented with 2.5 mg litre -1 kinetin + 0.2 mg litre-1 NAA. Rooting
was best on half strength MS medium supplemented with 1.0 mg litre-1
NAA. |
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